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Literature summary extracted from

  • Kadowaki, M.K.; Polizeli, M.L.; Terenzi, H.F.; Jorge, J.A.
    Characterization of trehalase activities from the thermophilic fungus Scytalidium thermophilum (1996), Biochim. Biophys. Acta, 1291, 199-205 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.28 AMP 10 mM, in presence of CaCl2, 18% residual activity for extracellular isoform, 12% residual activity, intracellular isoform. No activation in absence of Ca2+ Mycothermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 ADP 10 mM, 16% residual activity, extracellular isoform, 12% residual activity, intracellular isoform. In presence of CaCl2, 18% residual activity for extracellular isoform, 12% residual activity, intracellular isoform, respectively Mycothermus thermophilus
3.2.1.28 Al3+ 1 mM, no residual activity, both isoforms Mycothermus thermophilus
3.2.1.28 ATP 10 mM, 12% residual activity, extracellular isoform, 6% residual activity, intracellular isoform. In presence of CaCl2, 2% residual activity for extracellular isoform, 10% residual activity, intracellular isoform Mycothermus thermophilus
3.2.1.28 EDTA 1 mM, 70% residual activity for extracellular isoform, 46% residual activity, intracellular isoform Mycothermus thermophilus
3.2.1.28 Hg2+ 1 mM, no residual activity, both isoforms Mycothermus thermophilus
3.2.1.28 Zn2+ 1 mM, 50% residual activity for extracellular isoform, not inhibitory for intracellular isoform Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 2.24
-
 alpha,alpha-trehalose intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus
3.2.1.28 3.09
-
 alpha,alpha-trehalose presence of Mn2+, intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus
3.2.1.28 3.58
-
 alpha,alpha-trehalose extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus
3.2.1.28 6.43
-
 alpha,alpha-trehalose presence of Ca2+, intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus
3.2.1.28 7.63
-
 alpha,alpha-trehalose presence of Mn2+, extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus
3.2.1.28 9.51
-
 alpha,alpha-trehalose presence of Ca2+, extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 extracellular
-
 Mycothermus thermophilus
-
-
3.2.1.28 intracellular
-
 Mycothermus thermophilus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Ca2+ 5 mM, 2.5fold stimulation, both isoforms Mycothermus thermophilus
3.2.1.28 Mn2+ 5 mM, 3fold stimulation, both isoforms Mycothermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 370000
-
 gel filtration, extracellular isoform Mycothermus thermophilus
3.2.1.28 392000
-
 gel filtration, intracellular isoform Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Mycothermus thermophilus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein carbohydrate content 81%, extracellular isoform, and 51%, intracellular isoform, respectively Mycothermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 1700
-
 extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus
3.2.1.28 3700
-
 intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O both isoforms are highly specific for trehalose Mycothermus thermophilus 2 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 pentamer 5 * 82000, extracellular isoform, 5 * 85000, intracellular isoform, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 60
-
 extracellular isoform Mycothermus thermophilus
3.2.1.28 65
-
 intracellular isoform Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 6
-
 both isoforms Mycothermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.28 Mycothermus thermophilus isoelectric focusing, intracellular isoform
-
 3.4
3.2.1.28 Mycothermus thermophilus isoelectric focusing, extracellular isoform
-
 3.7