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Literature summary extracted from

  • Birrell, J.A.; Laurich, C.; Reijerse, E.J.; Ogata, H.; Lubitz, W.
    Importance of hydrogen bonding in fine tuning the [2Fe-2S] cluster redox potential of HydC from Thermotoga maritima (2016), Biochemistry, 55, 4344-4355 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.1.4 expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
1.12.1.4 A85P the mutant has an altered reduction potential compared to the wild type enzyme Thermotoga maritima
1.12.1.4 V131N the mutant has an altered reduction potential compared to the wild type enzyme Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.4 H2 + NAD+ + oxidized ferredoxin Thermotoga maritima
-
 H+ + NADH + reduced ferredoxin
-
 ?
1.12.1.4 H2 + NAD+ + oxidized ferredoxin Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
 H+ + NADH + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.4 Thermotoga maritima
-
-
-
1.12.1.4 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.4 streptactin column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.4 H2 + NAD+ + oxidized ferredoxin
-
 Thermotoga maritima H+ + NADH + reduced ferredoxin
-
 ?
1.12.1.4 H2 + NAD+ + oxidized ferredoxin
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 H+ + NADH + reduced ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.1.4 HydC gamma subunit Thermotoga maritima
1.12.1.4 NAD(P)+-linked [FeFe]-hydrogenase
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.4 [2Fe-2S]-center
-
 Thermotoga maritima