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Literature summary extracted from

  • Summitt, C.B.; Johnson, L.C.; Joensson, T.J.; Parsonage, D.; Holmes, R.P.; Lowther, W.T.
    Proline dehydrogenase 2 (PRODH2) is a hydroxyproline dehydrogenase (HYPDH) and molecular target for treating primary hyperoxaluria (2015), Biochem. J., 466, 273-281 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.5.5.3 medicine the enzyme is a therapeutic target for the treatment of primary hyperoxaluria Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.5.3 expressed in Escherichia coli C41(DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.5.3 (1R,3R)-3-hydroxy-cyclopentane-carboxylate
-
 Homo sapiens
1.5.5.3 5-hydroxy-1H-pyrazole-3-carboxylate
-
 Homo sapiens
1.5.5.3 menadione
-
 Homo sapiens
1.5.5.3 additional information (1S,3R)-3-hydroxy-cyclopentane carboxylic acid, trans-4-hydroxy-D-proline, cis-4-hydroxy-L-proline, and cis-4-hydroxy-D-proline do not cause inhibition even up to 5 mM Homo sapiens
1.5.5.3 Tetrahydrofuroic acid
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.5.3 0.025
-
 menaquinone at pH 7.5 and 25°C Homo sapiens
1.5.5.3 0.124
-
 coenzyme Q1 at pH 7.5 and 25°C Homo sapiens
1.5.5.3 0.143
-
 duroquinone at pH 7.5 and 25°C Homo sapiens
1.5.5.3 200
-
 trans-4-hydroxy-L-proline at pH 7.5 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.5.3 trans-4-hydroxy-L-proline + coenzyme Q10 Homo sapiens preferred substrate (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.3 Homo sapiens Q9UF12
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.3 Q-Sepharose column chromatography and HisPur cobalt resin column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.3 L-proline + coenzyme Q1
-
 Homo sapiens ?
-
 ?
1.5.5.3 additional information no activity with trans-4-hydroxy-D-proline, cis-4-hydroxy-L-proline, and cis-4-hydroxy-D-proline Homo sapiens ?
-
 ?
1.5.5.3 additional information the enzyme is a poor oxidase using O2 as electron acceptor Homo sapiens ?
-
 ?
1.5.5.3 trans-4-hydroxy-L-proline + coenzyme Q1
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
-
 ?
1.5.5.3 trans-4-hydroxy-L-proline + coenzyme Q10 preferred substrate Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
-
 ?
1.5.5.3 trans-4-hydroxy-L-proline + duroquinone
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + duroquinol
-
 ?
1.5.5.3 trans-4-hydroxy-L-proline + menadione
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menadiol
-
 ?
1.5.5.3 trans-4-hydroxy-L-proline + menaquinone
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menaquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.5.3 hydroxyproline oxidase
-
 Homo sapiens
1.5.5.3 HYPDH
-
 Homo sapiens
1.5.5.3 ProDH2
-
 Homo sapiens
1.5.5.3 proline dehydrogenase 2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.5.3 0.19
-
 coenzyme Q1 at pH 7.5 and 25°C Homo sapiens
1.5.5.3 0.19
-
 trans-4-hydroxy-L-proline at pH 7.5 and 25°C Homo sapiens
1.5.5.3 0.27
-
 duroquinone at pH 7.5 and 25°C Homo sapiens
1.5.5.3 0.28
-
 menaquinone at pH 7.5 and 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.5.3 FAD
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.5.3 0.306
-
 menadione at pH 7.5 and 25°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.5.3 1.5
-
 at pH 7.5 and 25°C Homo sapiens Tetrahydrofuroic acid
1.5.5.3 2.1
-
 at pH 7.5 and 25°C Homo sapiens (1R,3R)-3-hydroxy-cyclopentane-carboxylate
1.5.5.3 2.9
-
 at pH 7.5 and 25°C Homo sapiens 5-hydroxy-1H-pyrazole-3-carboxylate

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.5.3 0.93
-
 trans-4-hydroxy-L-proline at pH 7.5 and 25°C Homo sapiens
1.5.5.3 1.5
-
 coenzyme Q1 at pH 7.5 and 25°C Homo sapiens
1.5.5.3 1.9
-
 duroquinone at pH 7.5 and 25°C Homo sapiens
1.5.5.3 11
-
 menaquinone at pH 7.5 and 25°C Homo sapiens
1.5.5.3 75
-
 L-proline at pH 7.5 and 25°C Homo sapiens