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Literature summary extracted from

  • Luka, Z.; Pakhomova, S.; Loukachevitch, L.V.; Newcomer, M.E.; Wagner, C.
    Folate in demethylation the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate (2014), Biochem. Biophys. Res. Commun., 449, 392-398 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.8.4 crystal structure of the recombinant mature and precursor forms of enzyme and the enzyme complexed with 5,6,7,8-tetrahydrofolate. Both forms reveal similar kinetic parameters and have the same tertiary structure fold with two domains formed by N- and C-terminal halves of the protein. The active center is located in the N-terminal domain while the tetrahydrofolate binding site is located in the C-terminal domain about 40 A from the isoalloxazine ring of FAD. The folate binding site is connected with the enzyme active center via an intramolecular channel. This suggests the possible transfer of the intermediate imine of dimethylglycine from the active center to the bound 5,6,7,8-tetrahydrofolate where they can react producing a 5,10-methylenetetrahydrofolate Rattus norvegicus
1.5.8.4 recombinant mature and precursor forms, in complex with tetrahydrofolate. Both forms of DMGDH reveal similar kinetic parameters and have the same tertiary structure fold with two domains formed by N- and C-terminal halves of the protein. The active center is located in the N-terminal domain while the THF binding site is located in the C-terminal domain about 40 A from the isoalloxazine ring of FAD. The folate binding site is connected with the enzyme active center via an intramolecular channel Rattus norvegicus
1.5.8.4 using 0.2 M K/Na-tartrate-20% (w/v) PEG 3350 for the mature enzyme or 0.2 M NaSCN-20% (w/v) PEG 3350-0.1 M Tris pH 7.5 for the precursor Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.8.4 mitochondrion
-
Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.4 N,N-dimethylglycine + electron-transfer flavoprotein + H2O Rattus norvegicus
-
sarcosine + formaldehyde + reduced electron-transfer flavoprotein
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.4 Rattus norvegicus
-
-
-
1.5.8.4 Rattus norvegicus Q5RKL4
-
-
1.5.8.4 Rattus norvegicus Q63342
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.4 N,N-dimethylglycine + electron-transfer flavoprotein + H2O
-
Rattus norvegicus sarcosine + formaldehyde + reduced electron-transfer flavoprotein
-
?
1.5.8.4 N,N-dimethylglycine + oxidized ferricenium hexafluorophosphate + H2O
-
Rattus norvegicus sarcosine + formaldehyde + reduced ferricenium hexafluorophosphate + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.5.8.4 ? x * 96000, calculated from amino acid sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.5.8.4 DMGDH
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.4 FAD
-
Rattus norvegicus
1.5.8.4 tetrahydrofolate
-
Rattus norvegicus