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Literature summary extracted from

  • Kelly, C.; OMahony, M.; Fogarty, W.
    A novel thermostable extracellular beta-galactosidase of Paecilomyces varioti (1988), Appl. Microbiol. Biotechnol., 27, 383-388 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ba2+ 10 mM, 72% residual activity Paecilomyces variotii
3.2.1.23 Cu2+ 10 mM, 72% residual activity Paecilomyces variotii
3.2.1.23 D-glucono-delta-lactone mixed type inhibition Paecilomyces variotii
3.2.1.23 Fe2+ 10 mM, 80% residual activity Paecilomyces variotii
3.2.1.23 Hg2+ 10 mM, no residual activity Paecilomyces variotii
3.2.1.23 additional information not inhibitory: D-glucose at 0.5 mM, D-galactose at 20 mM Paecilomyces variotii
3.2.1.23 Ni2+ 10 mM, 72% residual activity Paecilomyces variotii
3.2.1.23 p-hydroxymercuribenzoate 1 mM, no residual activity Paecilomyces variotii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 64
-
 lactose pH 3.5, 40°C Paecilomyces variotii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.23 extracellular
-
 Paecilomyces variotii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 94000
-
 gel filtration Paecilomyces variotii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Paecilomyces variotii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Paecilomyces variotii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 1410
-
 substrate lactose, pH 3.5, 40°C Paecilomyces variotii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paecilomyces variotii 2-nitrophenol + D-galactopyranose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paecilomyces variotii 2-nitrophenol + D-glucopyranoside
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paecilomyces variotii 4-nitrophenol + D-galactopyranose
-
 ?
3.2.1.23 cellobiose + H2O 41.7% of the activtiy with lactose Paecilomyces variotii 2 D-glucose
-
 ?
3.2.1.23 gentiobiose + H2O reaction of EC 3.2.1.21, 14.3% of the activtiy with lactose Paecilomyces variotii 2 beta-D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Paecilomyces variotii D-galactose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 monomer 1 * 94000, SDS-PAGE Paecilomyces variotii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Paecilomyces variotii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 60
-
 3 h, presence of 4% lactose, 95% residual activity Paecilomyces variotii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 3.5
-
-
 Paecilomyces variotii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.23 Paecilomyces variotii isoelectric focusing
-
 4.1

Expression

EC Number Organism Comment Expression
3.2.1.23 Paecilomyces variotii submerged fermentation leads to levels of 73.8 units/ml at 72 h up