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Literature summary extracted from

  • Aalbers, F.S.; Fraaije, M.W.
    Coupled reactions by coupled enzymes alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions (2017), Appl. Microbiol. Biotechnol., 101, 7557-7565 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.13.22 synthesis a fusion protein that can convert cyclohexanol to epsilon-caprolactone in vitro is created. The alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct is expressed in Escherichia coli cells. By circumventing substrate and product inhibition, a more than 99% conversion of 200 mM cyclohexanol can be achieved in 24 h, with more than 13000 turnovers per fusion enzyme molecule Thermocrispum municipale

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.22 alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct expressed in Escherichia coli cells Thermocrispum municipale

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Thermocrispum municipale A0A1L1QK39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.22 alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct expressed in Escherichia coli cells Thermocrispum municipale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + H+ + O2
-
 Thermocrispum municipale hexano-6-lactone + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 CHMO
-
 Thermocrispum municipale