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Literature summary extracted from

  • Brock, B.J.; Rieble, S.; Gold, M.H.
    Purification and Ccaracterization of a 1,4-benzoquinone reductase from the basidiomycete Phanerochaete chrysosporium (1995), Appl. Environ. Microbiol., 61, 3076-3081 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 Cibacron blue 3GA
-
 Phanerodontia chrysosporium
1.6.5.2 dicumarol
-
 Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0024
-
 2-methoxy-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 0.0074
-
 2,6-dimethoxy-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 0.0105
-
 2,6-dichlorophenolindophenol pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 0.0208
-
 2,5-di-tert-butyl-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 0.032
-
 menadione pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 0.048
-
 NADPH pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 0.055
-
 NADH pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 44000
-
 gel filtration Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Phanerodontia chrysosporium Q9Y763
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2
-
 Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 217
-
 pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 2,5-di-tert-butyl-1,4-benzoquinone + NADH
-
 Phanerodontia chrysosporium 2,5-di-tert-butyl-1,4-benzoquinol + NAD+
-
 ?
1.6.5.2 2,6-dichlorophenolindophenol + NADPH
-
 Phanerodontia chrysosporium reduced 2,6-dichlorophenolindophenol + NADP+
-
 ?
1.6.5.2 2,6-dimethoxy-1,4-benzoquinone + NADH
-
 Phanerodontia chrysosporium 2,6-dimethoxy-1,4-benzoquinol + NAD+
-
 ?
1.6.5.2 2-methoxy-1,4-benzoquinone + NADPH
-
 Phanerodontia chrysosporium 2-methoxy-1,4-benzoquinol + NADP+
-
 ?
1.6.5.2 menadione + NADH
-
 Phanerodontia chrysosporium reduced menadione + NAD+
-
 ?
1.6.5.2 additional information catalyzes the two-electron reduction of several quinones and other electron acceptors utilizing either NADH or NADPH as an electron donor Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 dimer 2 * 22000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 1,4-benzoquinone reductase
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.5.2 30
-
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.2 330
-
 2,6-dichlorophenolindophenol pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 560
-
 2,6-dimethoxy-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 760
-
 NADPH pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 800
-
 NADH pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 1400
-
 2,5-di-tert-butyl-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 4900
-
 menadione pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 440000
-
 2-methoxy-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 5 6.5
-
 Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FMN absoprtion maxima at 375 and 450 nm Phanerodontia chrysosporium
1.6.5.2 NADH
-
 Phanerodontia chrysosporium
1.6.5.2 NADPH
-
 Phanerodontia chrysosporium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.6.5.2 Phanerodontia chrysosporium isoelectric focusing
-
 4.3

Expression

EC Number Organism Comment Expression
1.6.5.2 Phanerodontia chrysosporium expressed in cultures grown under both nitrogen-sufficient and nitrogen-limiting conditions up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6.5.2 15
-
 NADH pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 16
-
 NADPH pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 31
-
 2,6-dichlorophenolindophenol pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 67
-
 2,5-di-tert-butyl-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 150
-
 menadione pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 760
-
 2,6-dimethoxy-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium
1.6.5.2 180000
-
 2-methoxy-1,4-benzoquinone pH 6.0, temperature not specified in the publication Phanerodontia chrysosporium