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Literature summary extracted from

  • Coulter, C.; Kennedy, J.T.; McRoberts, W.C.; Harper, D.B.
    Purification and properties of an S-adenosylmethionine 2,4-disubstituted phenol O-methyltransferase from Phanerochaete chrysosporium (1993), Appl. Environ. Microbiol., 59, 706-711 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.25 Ag+ 0.1 mM, no resiudal activity Phanerodontia chrysosporium
2.1.1.25 Cu2+ 0.1 mM, no resiudal activity Phanerodontia chrysosporium
2.1.1.25 Hg2+ 0.1 mM, no resiudal activity Phanerodontia chrysosporium
2.1.1.25 additional information not inhibitory: EDTA, Mg2+, Ca2+, iodoacetamide Phanerodontia chrysosporium
2.1.1.25 Phenylmercuriacetate 0.01 mM, no resiudal activity Phanerodontia chrysosporium
2.1.1.25 S-adenosyl-L-homocysteine product inhibition Phanerodontia chrysosporium
2.1.1.25 Zn2+ 0.1 mM, 45% resiudal activity Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.25 0.034
-
4-hydroxy-3-methoxyacetophenone pH 8.0, 37°C Phanerodontia chrysosporium
2.1.1.25 0.099
-
S-adenosyl-L-methionine pH 8.0, 37°C Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.25 52000
-
gel filtration Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.25 Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.25
-
Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.25 101
-
pH 8.0, 37°C Phanerodontia chrysosporium

Storage Stability

EC Number Storage Stability Organism
2.1.1.25 -4°C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, 75% loss of activity, in presence of 10% glycerol 5% loss of activtiy Phanerodontia chrysosporium
2.1.1.25 2°C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, a half-life of approximately 30 h Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.25 additional information 3-methoxy- and 3,5-dimethoxy-substituted 4-hydroxy-benzaldehydes, -benzoic acids, and -acetophenones are the preferred substrates. The corresponding 3,4-dihydroxy compounds are methylated relatively slowly, while the 3-hydroxy-4-methoxy compounds are almost inactive as substrates. Substituents in both the 2 and 4 positions relative to the hydroxyl group appear to be essential for significant enzyme attack of a substrate. Xenobiotic compounds such as 2,4-dichlorophenol and 2,4-dibromophenol are methylated almost as readily as 4-hydroxy-3-methoxyacetophenone. No substrates: homovanillic acid, caffeic acid, ferulic acid Phanerodontia chrysosporium ?
-
?
2.1.1.25 S-adenosyl-L-methionine + 2,4-dibromophenol
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 2,4-dibromo-1-methoxybenzene
-
?
2.1.1.25 S-adenosyl-L-methionine + 2,4-dichlorophenol
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 2,4-dichloro-1-methoxybenzene
-
?
2.1.1.25 S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxyacetophenone
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 3,4,5-trimethoxyacetophenone
-
?
2.1.1.25 S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzaldehyde
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzaldehyde
-
?
2.1.1.25 S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzoic acid
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzoic acid
-
?
2.1.1.25 S-adenosyl-L-methionine + 4-hydroxy-3-methoxyacetophenone
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 3,4-dimethoxyacetophenone
-
?
2.1.1.25 S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzaldehyde
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 3,4-dimethoxybenzaldehyde
-
?
2.1.1.25 S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzoic acid
-
Phanerodontia chrysosporium S-adenosyl-L-homocysteine + 3,4-dimethoxybenzoic acid
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.25 monomer 1 * 54000, SDS-PAGE Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.25 55
-
rapid inactivation above Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.25 7 9
-
Phanerodontia chrysosporium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.25 0.041
-
S-adenosyl-L-homocysteine pH 8.0, 37°C Phanerodontia chrysosporium