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Literature summary extracted from

  • Li, Q.; Pei, J.; Zhao, L.; Xie, J.; Cao, F.; Wang, G.
    Overexpression and characterization of laccase from Trametes versicolor in Pichia pastoris (2014), Appl. Biochem. Microbiol., 50, 140-147 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 overexpression in Pichia pastoris Trametes versicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Fe2+
-
 Trametes versicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.43
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 55°C Trametes versicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Al3+ significantly enhances activity Trametes versicolor
1.10.3.2 Cu2+ significantly enhances activity Trametes versicolor

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes versicolor I6QS85
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant enzyme Trametes versicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 28.8
-
 pH 4.5, 55°C, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trametes versicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes versicolor ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 58000, SDS-PAGE Trametes versicolor

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 LccA
-
 Trametes versicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trametes versicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 30 80 30°C: about 70% of maximal activity, 80°C: about 45% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trametes versicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 30 50 2 h, stable Trametes versicolor
1.10.3.2 60
-
 1 h, residual activity is more that 50% Trametes versicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Trametes versicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2 7 6 h, 25°C, in the absence of the substrate, recombinant laccase is stable Trametes versicolor