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Literature summary extracted from

  • Feng, H.; Zhang, D.; Sun, Y.; Zhi, Y.; Mao, L.; Luo, Y.; Xu, L.; Wang, L.; Zhou, P.
    Expression and characterization of a recombinant laccase with alkalistable and thermostable properties from Streptomyces griseorubens JSD-1 (2015), Appl. Biochem. Biotechnol., 176, 547-562 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme alone can decolourize indigo carmine partially after 60-min incubation at 45 °C. Decolorization is much more efficient in the presence of syringaldehyde. Nearly 90 % decolorization is observed within 20 min Streptomyces griseorubens
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme alone can decolorize indigo carmine partially after 60-min incubation at 45°C. Decolorization is much more efficient in the presence of syringaldehyde. Nearly 90 % decolorization is observed within 20 min Streptomyces griseorubens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression in Escherichia coli Streptomyces griseorubens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 CdCl2 1 mM, 24.6% inhibition Streptomyces griseorubens
1.10.3.2 CoCl2 1 mM, 20.8% inhibition Streptomyces griseorubens
1.10.3.2 dithiothreitol 1 mM, 10% loss of activity Streptomyces griseorubens
1.10.3.2 EDTA 1 mM, 62% loss of activity Streptomyces griseorubens
1.10.3.2 HgCl2 1 mM, 33.3% inhibition Streptomyces griseorubens
1.10.3.2 NaN3 1 mM, 5.5% loss of activity Streptomyces griseorubens
1.10.3.2 SDS 1 mM, 20% loss of activity Streptomyces griseorubens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Streptomyces griseorubens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 CuCl2 1 mM, increases activity by 28.5% Streptomyces griseorubens
1.10.3.2 MnCl2 1 mM, increases activity by 11.7% Streptomyces griseorubens

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Streptomyces griseorubens
-
-
-
1.10.3.2 Streptomyces griseorubens JSD-1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Streptomyces griseorubens ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Streptomyces griseorubens JSD-1 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Streptomyces griseorubens ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Streptomyces griseorubens JSD-1 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Streptomyces griseorubens ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Streptomyces griseorubens JSD-1 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Streptomyces griseorubens ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Streptomyces griseorubens JSD-1 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 30
-
 8 h, 40% loss of activity Streptomyces griseorubens
1.10.3.2 40
-
 8 h, 60% loss of activity Streptomyces griseorubens
1.10.3.2 50
-
 8 h, 70% loss of activity Streptomyces griseorubens
1.10.3.2 60
-
 8 h, 90% loss of activity Streptomyces griseorubens
1.10.3.2 70
-
 8 h, complete loss of activity Streptomyces griseorubens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.058
-
 2,6-dimethoxyphenol 25°C, pH 9.0 Streptomyces griseorubens
1.10.3.2 0.337
-
 syringaldazine 25°C, pH 8.0 Streptomyces griseorubens
1.10.3.2 7.7
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 25°C, pH 4.0 Streptomyces griseorubens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Streptomyces griseorubens
1.10.3.2 8
-
 substrate: syringaldazine Streptomyces griseorubens
1.10.3.2 9
-
 substrate: 2,6-dimethoxyphenol Streptomyces griseorubens
1.10.3.2 10
-
 substrate: guaiacol Streptomyces griseorubens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3
-
 complete loss of activity within 1 day Streptomyces griseorubens
1.10.3.2 4 5 enzyme loses 50% activity within 2 days Streptomyces griseorubens
1.10.3.2 7 11 the enzyme retains over 50% residual activity after 5-day incubation Streptomyces griseorubens
1.10.3.2 10
-
 highest stability is observed at pH 10.0 with an approximately residual activity of 90% after 5 days Streptomyces griseorubens

Expression

EC Number Organism Comment Expression
1.10.3.2 Streptomyces griseorubens expression levels were are upregulated with the stimulation of rice straw or lignin. However, it iss expressed in relative low levels when induced by cellulose, xylan, pectin or glucose up