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Literature summary extracted from

  • Ou, Q.; Liu, Y.; Deng, J.; Chen, G.; Yang, Y.; Shen, P.; Wu, B.; Jiang, C.
    A novel D-amino acid oxidase from a contaminated agricultural soil metagenome and its characterization (2015), Antonie van Leeuwenhoek, 107, 1615-1623 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli Tunner(DE3) cells uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 Al3+
-
 uncultured bacterium
1.4.3.3 Ba2+
-
 uncultured bacterium
1.4.3.3 EDTA
-
 uncultured bacterium
1.4.3.3 SDS
-
 uncultured bacterium
1.4.3.3 Zn2+
-
 uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 2.96
-
 D-Met at pH 8.0 and 37°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.3 Cu2+
-
 uncultured bacterium
1.4.3.3 Fe2+
-
 uncultured bacterium
1.4.3.3 Mn2+
-
 uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 uncultured bacterium A0A0E3JXD9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 Ni-NTA column chromatography uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 4-fluoro-D-Phe + H2O + O2 82% activity compared to D-Met uncultured bacterium 4-fluorophenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Arg + H2O + O2 76% activity compared to D-Met uncultured bacterium 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-His + H2O + O2 12% activity compared to D-Met uncultured bacterium 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-Leu + H2O + O2 26% activity compared to D-Met uncultured bacterium 4-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Lys + H2O + O2 90% activity compared to D-Met uncultured bacterium 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-Met + H2O + O2 100% activity uncultured bacterium 4-methylthio-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Orn + H2O + O2 34% activity compared to D-Met uncultured bacterium 5-amino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Phe + H2O + O2 51% activity compared to D-Met uncultured bacterium phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Tyr + H2O + O2 11.6% activity compared to D-Met uncultured bacterium 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 additional information D-allo-isoleucine, D-valine, O-methyl-DL-serine, D-alanine, and D-proline are no substrates uncultured bacterium ?
-
 ?
1.4.3.3 N-methyl-DL-Leu + H2O + O2 5.8% activity compared to D-Met uncultured bacterium 4-methyl-2-oxopentanoate + methylamine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 ? x * 45000, SDS-PAGE uncultured bacterium
1.4.3.3 ? x * 44800, calculated from amino acid sequence uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 DAAO
-
 uncultured bacterium
1.4.3.3 DaoE
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.3 37
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.3 30 48 the enzyme shows 65 % of its maximum activity at a temperature below 30°C and 80 % of its maximum activity from 35 to 48°C uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 35 45 when temperature exceeds 35°C, the recombinant enzyme dramatically loses ist activity after 15 min of incubation. When incubated over 45°C for 5 min, the enzyme maintains about 30 % residual activity uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 8
-
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.3 7 9
-
 uncultured bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 uncultured bacterium