EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.19 | expressed in Trichoderma reesei | Pleurotus sapidus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 0.024 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 0.099 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 3.8 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 0.713 | - |
guaiacol | at pH 4.5 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 1.227 | - |
2,6-dimethoxyphenol | at pH 4.5 and 30°C | Pleurotus sapidus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.19 | 115000 | - |
gel filtration | Pleurotus sapidus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.19 | Reactive Blue 5 + H2O2 | Pleurotus sapidus | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
1.11.1.19 | Reactive Blue 5 + H2O2 | Pleurotus sapidus DSM 8266 | - |
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.19 | Pleurotus sapidus | A0A0F7VJ89 | - |
- |
1.11.1.19 | Pleurotus sapidus DSM 8266 | A0A0F7VJ89 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.11.1.19 | glycoprotein | - |
Pleurotus sapidus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.19 | phenyl-Sepharose column chromatography and SP Sepharose 200 column chromatography | Pleurotus sapidus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.19 | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pleurotus sapidus | ? | - |
? | |
1.11.1.19 | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pleurotus sapidus DSM 8266 | ? | - |
? | |
1.11.1.19 | 2,6-dimethoxyphenol + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
1.11.1.19 | 2,6-dimethoxyphenol + H2O2 + H+ | - |
Pleurotus sapidus DSM 8266 | ? | - |
? | |
1.11.1.19 | annatto + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
1.11.1.19 | beta-carotene + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
1.11.1.19 | guaiacol + H2O2 + H+ | - |
Pleurotus sapidus | ? | - |
? | |
1.11.1.19 | guaiacol + H2O2 + H+ | - |
Pleurotus sapidus DSM 8266 | ? | - |
? | |
1.11.1.19 | additional information | the enzyme has an oxidase function in addition to a peroxidase activity | Pleurotus sapidus | ? | - |
? | |
1.11.1.19 | additional information | the enzyme has an oxidase function in addition to a peroxidase activity | Pleurotus sapidus DSM 8266 | ? | - |
? | |
1.11.1.19 | Reactive Blue 5 + H2O2 | - |
Pleurotus sapidus | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? | |
1.11.1.19 | Reactive Blue 5 + H2O2 | - |
Pleurotus sapidus DSM 8266 | phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.19 | homodimer | 2 * 57100, calculated from amino acid sequence | Pleurotus sapidus |
1.11.1.19 | homodimer | 2 * 57600, SDS-PAGE | Pleurotus sapidus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.19 | dye-decolorizing peroxidase | - |
Pleurotus sapidus |
1.11.1.19 | DyP | - |
Pleurotus sapidus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.19 | 15 | 70 | after 5 min incubation, respectively, the enzyme shows 100% activity between 15 and 25°C, about 85% activity at 30-35°C, about 75% activity at 40-45°C, about 62% activity at 50°C, and less than 30% activity at 55°C and above | Pleurotus sapidus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 18 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 60 | - |
2,6-dimethoxyphenol | at pH 4.5 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 74 | - |
guaiacol | at pH 4.5 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 375 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 3.8 and 30°C | Pleurotus sapidus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.19 | 3.5 | 4.5 | - |
Pleurotus sapidus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.11.1.19 | Pleurotus sapidus | calculated from amino acid sequence | - |
5.7 |
1.11.1.19 | Pleurotus sapidus | isoelectric focusing | - |
6.7 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 49 | - |
2,6-dimethoxyphenol | at pH 4.5 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 100 | - |
guaiacol | at pH 4.5 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 750 | - |
Reactive Blue 5 | at pH 4.0 and 30°C | Pleurotus sapidus | |
1.11.1.19 | 3800 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 3.8 and 30°C | Pleurotus sapidus |