Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Doan, T.T.; Carlsson, A.S.; Stymne, S.; Hofvander, P.
    Biochemical characteristics of AtFAR2, a fatty acid reductase from Arabidopsis thaliana that reduces fatty acyl-CoA and -ACP substrates into fatty alcohols (2016), Acta Biochim. Pol., 63, 565-570 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.42 gene FAR2, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), transient expression of GFP-tagged FAR2 in Nicotiana benthamiana leaves under control of CaMV 35S promoter via Agrobacterium tumefaciens strain V3101::pMP90 transfection method Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.42 0.00531
-
 hexadecanal pH 7.0, 30°C, recombinant enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.42 chloroplast enzyme AtFAR2 is targeted to chloro­plasts Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.42 hexadecanal + CoA + NAD+ Arabidopsis thaliana
-
 hexadecanoyl-CoA + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.42 Arabidopsis thaliana Q08891
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.42 recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.42 hexadecanal + CoA + NAD+
-
 Arabidopsis thaliana hexadecanoyl-CoA + NADH + H+
-
 ?
1.2.1.42 additional information Arabidopsis thaliana AtFAR2 has the ability to use both, C16:0-ACP and C16:0-CoA substrates for the production of C16:0- alcohol, purified enzyme AtFAR2 is specific for acyl-ACP substrates, AtFAR2 shows the high­est activity towards C16:0-CoA, approximately 11times higher in comparison to the activity towards C18:0-CoA. AtFAR2 displays a very low activity with C18:1-CoA, and application of C14:0-CoA as a substrate generates only aldehyde and free fatty acid Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.42 AtFAR2
-
 Arabidopsis thaliana
1.2.1.42 FAR2
-
 Arabidopsis thaliana
1.2.1.42 MS2
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.42 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.42 additional information
-
 low activity with alcohols and free fatty acids occurs at above pH 9.0 Arabidopsis thaliana
1.2.1.42 6
-
 with aldehyde substrates Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.42 NAD+
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.2.1.42 evolution AtFAR2 is highly similar in properties and substrate specificity to AtFAR6 Arabidopsis thaliana
1.2.1.42 malfunction mutations in specific genes encoding fatty acid reductases (FAR) re­sponsible for fatty alcohol production cause abnormal development of pollen. A disrupted AtFAR2 (MS2) gene in Arabidopsis thaliana results in pollen developing an abnormal exine layer and a reduced fertility pheno­type Arabidopsis thaliana
1.2.1.42 physiological function AtFAR2 is a fatty acid reductase from Arabidopsis thaliana that reduces fatty acyl-CoA and -ACP substrates into fatty alcohols, cf. EC 1.2.1.80. Enzyme AtFAR2 is the major enzyme respon­sible for exine layer functionality, AtFAR6 might provide functional redundancy to AtFAR2 Arabidopsis thaliana