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Literature summary extracted from

  • Kim, S.H.; Wang, Y.; Khomutov, M.; Khomutov, A.; Fuqua, C.; Michael, A.J.
    The essential role of spermidine in growth of Agrobacterium tumefaciens is determined by the 1,3-diaminopropane moiety (2016), ACS Chem. Biol., 11, 491-499 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.43 L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+ Agrobacterium tumefaciens
-
 carboxynorspermidine + H2O + NADP+ ? ?
1.5.1.43 L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+ Agrobacterium tumefaciens C58 / ATCC 33970
-
 carboxynorspermidine + H2O + NADP+ ? ?
1.5.1.43 L-aspartate 4-semialdehyde + putrescine + NADPH + H+ Agrobacterium tumefaciens
-
 carboxyspermidine + H2O + NADP+ ? ?
1.5.1.43 L-aspartate 4-semialdehyde + putrescine + NADPH + H+ Agrobacterium tumefaciens C58 / ATCC 33970
-
 carboxyspermidine + H2O + NADP+ ? ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.43 Agrobacterium tumefaciens A9CG66
-
-
1.5.1.43 Agrobacterium tumefaciens C58 / ATCC 33970 A9CG66
-
-
4.1.1.96 Agrobacterium tumefaciens A9CG65
-
-
4.1.1.96 Agrobacterium tumefaciens C58 A9CG65
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.43 L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
 Agrobacterium tumefaciens carboxynorspermidine + H2O + NADP+ ? ?
1.5.1.43 L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
 Agrobacterium tumefaciens C58 / ATCC 33970 carboxynorspermidine + H2O + NADP+ ? ?
1.5.1.43 L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
 Agrobacterium tumefaciens carboxyspermidine + H2O + NADP+ ? ?
1.5.1.43 L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
 Agrobacterium tumefaciens C58 / ATCC 33970 carboxyspermidine + H2O + NADP+ ? ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.43 Atu4170
-
 Agrobacterium tumefaciens
1.5.1.43 carboxyspermidine dehydrogenase
-
 Agrobacterium tumefaciens
1.5.1.43 CASDH
-
 Agrobacterium tumefaciens
4.1.1.96 nspC
-
 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.43 NADPH
-
 Agrobacterium tumefaciens

General Information

EC Number General Information Comment Organism
1.5.1.43 malfunction the DELTACASDH (Atu4170) mutant strain accumulates putrescine and homospermidine but lacks spermidine. The DELTA4170 strain contains endogenously produced putrescine and homospermidine, but there is no growth of this strain in the absence of exogenously supplied polyamines. Homospermidine biosynthesis is induced only after spermidine depletion. The DELTACASDH strain grows well in medium with added exogenous N8-acetylspermidine Agrobacterium tumefaciens
1.5.1.43 metabolism when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Spermidine represses spermine biosynthesis, but when spermidine levels decrease, it is then converted by carboxyspermidine dehydrogenase and decarboxylase enzymes to spermine, which is resistant to retroconversion and constitutes a sequestered pool of protected 1,3-diaminopropane modules required for growth. Polyamine biosynthesis in Agrobacterium tumefaciens strain C58, overview Agrobacterium tumefaciens
1.5.1.43 physiological function when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Essential role of a terminal 1,3-diaminopropane moiety in growth of Agrobacterium tumefaciens Agrobacterium tumefaciens
4.1.1.96 physiological function identification of a spermidine retroconversion activity in bacteria, producing diamine putrescine from triamine spermidine. When spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase which produce spermidine from putrescine. A strain lacking carboxyspermidine decarboxylase activity accumulates carboxyspermidine, some putrescine and a small amount of homospermidine Agrobacterium tumefaciens