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The essential role of spermidine in growth of Agrobacterium tumefaciens is determined by the 1,3-diaminopropane moiety

Kim, S.H.; Wang, Y.; Khomutov, M.; Khomutov, A.; Fuqua, C.; Michael, A.J.; ACS Chem. Biol. 11, 491-499 (2016)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens
-
carboxynorspermidine + H2O + NADP+
?
-
?
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxynorspermidine + H2O + NADP+
?
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens
-
carboxyspermidine + H2O + NADP+
?
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxyspermidine + H2O + NADP+
?
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.1.43
Agrobacterium tumefaciens
A9CG66
-
-
1.5.1.43
Agrobacterium tumefaciens C58 / ATCC 33970
A9CG66
-
-
4.1.1.96
Agrobacterium tumefaciens
A9CG65
-
-
4.1.1.96
Agrobacterium tumefaciens C58
A9CG65
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxynorspermidine + H2O + NADP+
?
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxynorspermidine + H2O + NADP+
?
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxyspermidine + H2O + NADP+
?
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxyspermidine + H2O + NADP+
?
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.43
NADPH
-
Agrobacterium tumefaciens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.43
NADPH
-
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens
-
carboxynorspermidine + H2O + NADP+
?
-
?
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxynorspermidine + H2O + NADP+
?
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens
-
carboxyspermidine + H2O + NADP+
?
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxyspermidine + H2O + NADP+
?
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxynorspermidine + H2O + NADP+
?
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxynorspermidine + H2O + NADP+
?
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxyspermidine + H2O + NADP+
?
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxyspermidine + H2O + NADP+
?
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.5.1.43
malfunction
the DELTACASDH (Atu4170) mutant strain accumulates putrescine and homospermidine but lacks spermidine. The DELTA4170 strain contains endogenously produced putrescine and homospermidine, but there is no growth of this strain in the absence of exogenously supplied polyamines. Homospermidine biosynthesis is induced only after spermidine depletion. The DELTACASDH strain grows well in medium with added exogenous N8-acetylspermidine
Agrobacterium tumefaciens
1.5.1.43
metabolism
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Spermidine represses spermine biosynthesis, but when spermidine levels decrease, it is then converted by carboxyspermidine dehydrogenase and decarboxylase enzymes to spermine, which is resistant to retroconversion and constitutes a sequestered pool of protected 1,3-diaminopropane modules required for growth. Polyamine biosynthesis in Agrobacterium tumefaciens strain C58, overview
Agrobacterium tumefaciens
1.5.1.43
physiological function
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Essential role of a terminal 1,3-diaminopropane moiety in growth of Agrobacterium tumefaciens
Agrobacterium tumefaciens
4.1.1.96
physiological function
identification of a spermidine retroconversion activity in bacteria, producing diamine putrescine from triamine spermidine. When spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase which produce spermidine from putrescine. A strain lacking carboxyspermidine decarboxylase activity accumulates carboxyspermidine, some putrescine and a small amount of homospermidine
Agrobacterium tumefaciens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.5.1.43
malfunction
the DELTACASDH (Atu4170) mutant strain accumulates putrescine and homospermidine but lacks spermidine. The DELTA4170 strain contains endogenously produced putrescine and homospermidine, but there is no growth of this strain in the absence of exogenously supplied polyamines. Homospermidine biosynthesis is induced only after spermidine depletion. The DELTACASDH strain grows well in medium with added exogenous N8-acetylspermidine
Agrobacterium tumefaciens
1.5.1.43
metabolism
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Spermidine represses spermine biosynthesis, but when spermidine levels decrease, it is then converted by carboxyspermidine dehydrogenase and decarboxylase enzymes to spermine, which is resistant to retroconversion and constitutes a sequestered pool of protected 1,3-diaminopropane modules required for growth. Polyamine biosynthesis in Agrobacterium tumefaciens strain C58, overview
Agrobacterium tumefaciens
1.5.1.43
physiological function
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Essential role of a terminal 1,3-diaminopropane moiety in growth of Agrobacterium tumefaciens
Agrobacterium tumefaciens
4.1.1.96
physiological function
identification of a spermidine retroconversion activity in bacteria, producing diamine putrescine from triamine spermidine. When spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase which produce spermidine from putrescine. A strain lacking carboxyspermidine decarboxylase activity accumulates carboxyspermidine, some putrescine and a small amount of homospermidine
Agrobacterium tumefaciens