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Literature summary extracted from
- Gene cloning and expression of a glucoside 3-dehydrogenase from Sphingobacterium faecium ZJF-D6, and used it to produce N-p-nitrophenyl-3-ketovalidamine (2017), World J. Microbiol. Biotechnol., 33, 21.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.99.13 | expressed in Escherichia coli BL21(DE3) cells | Sphingobacterium faecium |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 62900 | - |
- |
Sphingobacterium faecium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.13 | Sphingobacterium faecium | A0A140CUL9 | ZJF-D6 | - |
| 1.1.99.13 | Sphingobacterium faecium DSM 11690 | A0A140CUL9 | ZJF-D6 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.13 | Ni-NTA column chromatography | Sphingobacterium faecium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 0.4 | - |
crude extract, at pH 7.6 and 30°C | Sphingobacterium faecium |
| 1.1.99.13 | 2.56 | - |
after 6.41fold purification, at pH 7.6 and 30°C | Sphingobacterium faecium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.13 | 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | 43.2% activity compared to D-glucose | Sphingobacterium faecium | 3-dehydro-2-deoxy-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? |  |
| 1.1.99.13 | 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | 43.2% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | 3-dehydro-2-deoxy-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | alpha-methyl-D-glucoside + 2,6-dichlorophenolindophenol | 98.8% activity compared to D-glucose | Sphingobacterium faecium | methyl 3-dehydro-alpha-D-glucoside + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | alpha-methyl-D-glucoside + 2,6-dichlorophenolindophenol | 98.8% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | methyl 3-dehydro-alpha-D-glucoside + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | cellobiose + 2,6-dichlorophenolindophenol | 105.9% activity compared to D-glucose | Sphingobacterium faecium | 4-O-beta-D-xylo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | cellobiose + 2,6-dichlorophenolindophenol | 105.9% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | 4-O-beta-D-xylo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | D-fructose + 2,6-dichlorophenolindophenol | 36.5% activity compared to D-glucose | Sphingobacterium faecium | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | D-fructose + 2,6-dichlorophenolindophenol | 36.5% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | D-galactose + 2,6-dichlorophenolindophenol | 76.5% activity compared to D-glucose | Sphingobacterium faecium | 3-dehydro-D-galactose + 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | D-glucose + 2,6-dichlorophenolindophenol | 100% activity | Sphingobacterium faecium | 3-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | D-mannose + 2,6-dichlorophenolindophenol | 2.3% activity compared to D-glucose | Sphingobacterium faecium | 3-dehydro-D-mannose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | isomaltose + 2,6-dichlorophenolindophenol | 109.4% activity compared to D-glucose | Sphingobacterium faecium | 6-O-alpha-D-xylo-hexopyranosyl-4-ulose-alpha-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | lactose + 2,6-dichlorophenolindophenol | 113.5% activity compared to D-glucose | Sphingobacterium faecium | 4-O-beta-D-xylo-hexopyranosyl-4-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | maltose + 2,6-dichlorophenolindophenol | 123.5% activity compared to D-glucose | Sphingobacterium faecium | 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | maltose + 2,6-dichlorophenolindophenol | 123.5% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | N-p-nitrophenylvalidamine + 2,6-dichlorophenolindophenol | - |
Sphingobacterium faecium | N-p-nitrophenyl-3-ketovalidamine + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | sucrose + 2,6-dichlorophenolindophenol | 96.8% activity compared to D-glucose | Sphingobacterium faecium | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.13 | trehalose + 2,6-dichlorophenolindophenol | 141.2% activity compared to D-glucose | Sphingobacterium faecium | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.13 | ? | x * 62900, calculated from amino acid sequence | Sphingobacterium faecium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.13 | G3DH | - |
Sphingobacterium faecium |
| 1.1.99.13 | glucoside 3-dehydrogenase | - |
Sphingobacterium faecium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 30 | - |
- |
Sphingobacterium faecium |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 20 | 40 | more than 80% activity between 20 and 40°C, the activity decreases sharply with temperature above 35°C, and almost 60% of the activity remains at 50°C | Sphingobacterium faecium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 40 | 60 | After 180 min at 40°C, the enzyme shows more than 90% of the initial activity. Nearly 20% activity is lost after pre-incubation at 50°C for 180 min, and the halflife of the enzyme at 60 °C is about 170 min | Sphingobacterium faecium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 7.6 | - |
- |
Sphingobacterium faecium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 5.4 | 9.6 | more than 60% activity between pH 6.0 and 9.0. When pH is decreased to 5.4, about 35% of the initial activity is observed. When pH is increased to 9.6, about 40% of the relative activity remains | Sphingobacterium faecium |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 5.8 | 9 | after pre-incubation in different pH at 4°C for 24 h, the enzyme retains more than 60% of the initial enzyme activity between pH 5.8 and 9.0 | Sphingobacterium faecium |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.99.13 | Sphingobacterium faecium | calculated from amino acid sequence | - |
6.5 |
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