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Literature summary extracted from

  • Desai, K.K.; Beltrame, A.L.; Raines, R.T.
    Coevolution of RtcB and Archease created a multiple-turnover RNA ligase (2015), RNA, 21, 1866-1872.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.8 expression in Escherichia coli Thermobifida fusca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.8 Mn2+ assay in presence of Mn2+ Thermus thermophilus
6.5.1.8 Mn2+ assay in presence of Mn2+ Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.8 Thermobifida fusca
-
-
-
6.5.1.8 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.8 (ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP
-
 Thermus thermophilus (ribonucleotide)40 + GMP + diphosphate overall reaction ?
6.5.1.8 (ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP
-
 Thermobifida fusca (ribonucleotide)40 + GMP + diphosphate overall reaction, phosphodiester bond synthesis is rate limiting ?
6.5.1.8 additional information no cosubstrate: ATP Thermus thermophilus ?
-
 ?
6.5.1.8 additional information no cosubstrate: ATP Thermobifida fusca ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.8 0.0014
-
 (ribonucleotide)20-3'-phosphate pH 7.4, 45°C Thermobifida fusca
6.5.1.8 0.0043
-
 (ribonucleotide)20-3'-phosphate pH 7.4, 70°C Thermus thermophilus
6.5.1.8 0.0122
-
 (ribonucleotide)20-3'-phosphate pH 7.4, 70°C, presence of archease Thermus thermophilus

General Information

EC Number General Information Comment Organism
6.5.1.8 physiological function RtcB from Thermobifida fusca is a single-turnover enzyme that is unable to be converted into a multiple-turnover ligase by archease from other organisms Thermobifida fusca
6.5.1.8 physiological function RtcB from Thermus thermophilus displays a strong dependency on Archease for maximal activity and can catalyze multiple turnovers only in the presence of archease. Archease from P. horikoshii can activate Thermus thermophilus RtcB Thermus thermophilus