EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.127 | expressed in Escherichia coli BL21(DE3) cells | Pectobacterium carotovorum subsp. carotovorum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.127 | in complex with NAD+, hanging drop vapor diffusion method, using 5% (w/v) polyethylene glycol 4000, and 10% (v/v) isopropanol | Pectobacterium carotovorum subsp. carotovorum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.127 | 0.0021 | - |
NADH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum | |
1.1.1.127 | 0.005 | - |
NADPH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 25000 | - |
4 * 25000, SDS-PAGE | Pectobacterium carotovorum subsp. carotovorum |
1.1.1.127 | 90000 | - |
gel filtration | Pectobacterium carotovorum subsp. carotovorum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.127 | 2,5-didehydro-3-deoxy-D-gluconate + NADH + H+ | Pectobacterium carotovorum subsp. carotovorum | - |
2-dehydro-3-deoxy-D-gluconate + NAD+ | - |
? | |
1.1.1.127 | 2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+ | Pectobacterium carotovorum subsp. carotovorum | - |
2-dehydro-3-deoxy-D-gluconate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.127 | Pectobacterium carotovorum subsp. carotovorum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.127 | Toyopearl DEAE-650 M column chromatography, Toyopearl butyl-650 M column chromatography, Q-Sepharose column chromatography, and Superdex 200 pg gel filtration | Pectobacterium carotovorum subsp. carotovorum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.127 | 2,5-didehydro-3-deoxy-D-gluconate + NADH + H+ | - |
Pectobacterium carotovorum subsp. carotovorum | 2-dehydro-3-deoxy-D-gluconate + NAD+ | - |
? | |
1.1.1.127 | 2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+ | - |
Pectobacterium carotovorum subsp. carotovorum | 2-dehydro-3-deoxy-D-gluconate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.127 | homotetramer | 4 * 25000, SDS-PAGE | Pectobacterium carotovorum subsp. carotovorum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.127 | 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase | - |
Pectobacterium carotovorum subsp. carotovorum |
1.1.1.127 | 2-keto-3-deoxy-D-gluconate dehydrogenase | - |
Pectobacterium carotovorum subsp. carotovorum |
1.1.1.127 | KduD | - |
Pectobacterium carotovorum subsp. carotovorum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 45 | - |
- |
Pectobacterium carotovorum subsp. carotovorum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 40 | - |
the enzyme shows more than 70% activity at 40°C for 5 min | Pectobacterium carotovorum subsp. carotovorum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.127 | 0.021 | - |
NADPH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum | |
1.1.1.127 | 0.024 | - |
NADH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 7.2 | - |
- |
Pectobacterium carotovorum subsp. carotovorum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.127 | NADH | the enzyme utilizes both NADH and NADPH | Pectobacterium carotovorum subsp. carotovorum | |
1.1.1.127 | NADPH | the enzyme utilizes both NADH and NADPH | Pectobacterium carotovorum subsp. carotovorum |