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Literature summary extracted from

  • Nakagawa, H.; Kuratani, M.; Goto-Ito, S.; Ito, T.; Katsura, K.; Terada, T.; Shirouzu, M.; Sekine, S.; Shigi, N.; Yokoyama, S.
    Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA (2013), Proteins Struct. Funct. Bioinform., 81, 1232-1244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.15
-
 Thermus thermophilus
2.8.1.15
-
 Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.15 to 2.1 A resolution. TtuA forms a homodimer, and each subunit contains a catalytic domain and unique N- and C-terminal zinc fingers. Three conserved cysteine residues are clustered in the putative catalytic site Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.15 C130S about 10% of wild-type activity Thermus thermophilus
2.8.1.15 C133S no activity Thermus thermophilus
2.8.1.15 C222S about 5-10% of wild-type activity Thermus thermophilus
2.8.1.15 D161A no activity Thermus thermophilus
2.8.1.15 D59A no activity Thermus thermophilus
2.8.1.15 E203A about 20% of wild-type activity Thermus thermophilus
2.8.1.15 H157A about 80% of wild-type activity Thermus thermophilus
2.8.1.15 K58A about 70% of wild-type activity Thermus thermophilus
2.8.1.15 N158A about 75% of wild-type activity Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.15 Zinc TtuA has four conserved CXXC motifs and one CXXH motif. In the TtuA structure, the first zinc finger is made up of the first and second CXXC/H motifs, where the zinc atom is coordinated by Cys3, Cys6, Cys22, and His25. The second zinc finger is made up of the fourth and fifth CXXC motifs, where the zinc atom is coordinated by Cys272, Cys275, Cys284, and Cys287 Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.15 35600
-
-
 Pyrococcus horikoshii
2.8.1.15 70800
-
 analytical ultracentrifugation Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.15 Pyrococcus horikoshii O58038
-
-
2.8.1.15 Pyrococcus horikoshii DSM 12428 O58038
-
-
2.8.1.15 Thermus thermophilus Q72LF3
-
-
2.8.1.15 Thermus thermophilus DSM 7039 Q72LF3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Thermus thermophilus AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Pyrococcus horikoshii AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Pyrococcus horikoshii DSM 12428 AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Thermus thermophilus DSM 7039 AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.15 dimer 2 * 35600, calculated Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
2.8.1.15 PH0300
-
 Pyrococcus horikoshii
2.8.1.15 TtuA
-
 Thermus thermophilus
2.8.1.15 TtuA
-
 Pyrococcus horikoshii