EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.67 | expressed in Escherichia coli BLR(DE3) cells | Pseudomonas fluorescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.67 | D230A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | E133A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | E133Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | H303A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | H303A/R373A/K381A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | K295A | inactive | Pseudomonas fluorescens |
1.1.1.67 | K381A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | N191A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | N300A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | N300D | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | N300S | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
1.1.1.67 | R373A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.67 | 4.56 | - |
D-mannitol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 5.98 | - |
D-mannitol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 8.15 | - |
D-mannitol | mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 14.96 | - |
D-mannitol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 17.49 | - |
D-mannitol | mutant enzyme R373A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 18.21 | - |
D-mannitol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 20.36 | - |
D-arabitol | mutant enzyme D230A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 20.42 | - |
D-mannitol | mutant enzyme N300D, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 23.89 | - |
D-mannitol | mutant enzyme K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 33.15 | - |
D-arabitol | mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 42.74 | - |
D-mannitol | mutant enzyme D230A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 49.95 | - |
D-mannitol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 55.85 | - |
D-arabitol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 68.94 | - |
D-mannitol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 93.85 | - |
D-mannitol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 94.82 | - |
D-arabitol | mutant enzyme N300D, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 125.5 | - |
D-arabitol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 126.5 | - |
D-arabitol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 126.9 | - |
D-arabitol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 132.1 | - |
D-arabitol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 134.1 | - |
D-arabitol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 144.5 | - |
D-arabitol | mutant enzyme R373A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 145.1 | - |
D-arabitol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 176.9 | - |
D-arabitol | mutant enzyme K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 1800 | - |
meso-erythritol | Km far above 1800 mM, mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 1800 | - |
meso-erythritol | Km far above 1800 mM, mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 1800 | - |
meso-erythritol | Km far above 1800 mM, mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 1800 | - |
meso-erythritol | Km far above 1800 mM, mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 1800 | - |
meso-erythritol | Km far above 1800 mM, mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 1800 | - |
meso-erythritol | Km far above 1800 mM, mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 1800 | - |
meso-erythritol | Km far above 1800 mM, wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.67 | D-mannitol + NAD+ | Pseudomonas fluorescens | - |
D-fructose + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.67 | Pseudomonas fluorescens | O08355 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.67 | HisPur cobalt resin column chromatography | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.67 | D-arabitol + NAD+ | - |
Pseudomonas fluorescens | ? | - |
? | |
1.1.1.67 | D-mannitol + NAD+ | - |
Pseudomonas fluorescens | D-fructose + NADH + H+ | - |
r | |
1.1.1.67 | meso-erythritol + NAD+ | - |
Pseudomonas fluorescens | ? | - |
? | |
1.1.1.67 | additional information | no activity with glycerol, 1, 2-hexanediol, and 1,2,3-hexanetriol | Pseudomonas fluorescens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.67 | MDH | - |
Pseudomonas fluorescens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.67 | 0.00083 | - |
D-mannitol | mutant enzyme N300D, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.001 | - |
D-mannitol | mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0012 | - |
meso-erythritol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0013 | - |
D-arabitol | mutant enzyme N300D, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0017 | - |
D-arabitol | mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0024 | - |
meso-erythritol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0029 | - |
D-arabitol | mutant enzyme D230A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0036 | - |
meso-erythritol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0159 | - |
meso-erythritol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.018 | - |
D-mannitol | mutant enzyme R373A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.034 | - |
D-arabitol | mutant enzyme R373A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.044 | - |
D-arabitol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.06 | - |
D-arabitol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.092 | - |
D-arabitol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.096 | - |
D-mannitol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.19 | - |
D-mannitol | mutant enzyme K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.209 | - |
D-mannitol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.21 | - |
D-mannitol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.29 | - |
D-arabitol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.37 | - |
D-arabitol | mutant enzyme K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.39 | - |
D-mannitol | mutant enzyme D230A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.489 | - |
meso-erythritol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.49 | - |
D-mannitol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.538 | - |
meso-erythritol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.599 | - |
meso-erythritol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 3.22 | - |
D-mannitol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 3.99 | - |
D-mannitol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 4.39 | - |
D-mannitol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 5.5 | - |
D-arabitol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 6.59 | - |
D-arabitol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 7.3 | - |
D-arabitol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.67 | NAD+ | - |
Pseudomonas fluorescens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.67 | 0.0000021 | - |
meso-erythritol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0000044 | - |
meso-erythritol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0000065 | - |
meso-erythritol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.000014 | - |
D-arabitol | mutant enzyme N300D, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.000029 | - |
meso-erythritol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00004 | - |
D-mannitol | mutant enzyme N300D, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.000051 | - |
D-arabitol | mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00014 | - |
D-arabitol | mutant enzyme D230A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00016 | - |
D-mannitol | mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00024 | - |
D-arabitol | mutant enzyme R373A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00035 | - |
D-arabitol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00047 | - |
D-arabitol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00069 | - |
D-arabitol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00088 | - |
meso-erythritol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00097 | - |
meso-erythritol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00102 | - |
D-mannitol | mutant enzyme R373A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.0018 | - |
meso-erythritol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00192 | - |
D-mannitol | mutant enzyme N300S, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.002 | - |
D-arabitol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00206 | - |
D-arabitol | mutant enzyme K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00311 | - |
D-mannitol | mutant enzyme N300A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00458 | - |
D-mannitol | mutant enzyme N191A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00522 | - |
D-mannitol | mutant enzyme H303A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00775 | - |
D-mannitol | mutant enzyme K381A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.00908 | - |
D-mannitol | mutant enzyme D230A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.04332 | - |
D-arabitol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.05448 | - |
D-arabitol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.118 | - |
D-arabitol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.215 | - |
D-mannitol | mutant enzyme E133Q, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.219 | - |
D-mannitol | mutant enzyme E133A, at pH 7.4 and 25°C | Pseudomonas fluorescens | |
1.1.1.67 | 0.734 | - |
D-mannitol | wild type enzyme, at pH 7.4 and 25°C | Pseudomonas fluorescens |