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Literature summary extracted from

  • Lucas, J.; Siegel, J.
    Erratum: Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2-dehydrogenase (2015), Protein Sci., 24, 936-945.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.67 expressed in Escherichia coli BLR(DE3) cells Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.67 D230A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 E133A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 E133Q the mutant shows reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 H303A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 H303A/R373A/K381A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 K295A inactive Pseudomonas fluorescens
1.1.1.67 K381A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 N191A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 N300A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 N300D the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 N300S the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
1.1.1.67 R373A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.67 4.56
-
 D-mannitol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 5.98
-
 D-mannitol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 8.15
-
 D-mannitol mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 14.96
-
 D-mannitol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 17.49
-
 D-mannitol mutant enzyme R373A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 18.21
-
 D-mannitol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 20.36
-
 D-arabitol mutant enzyme D230A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 20.42
-
 D-mannitol mutant enzyme N300D, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 23.89
-
 D-mannitol mutant enzyme K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 33.15
-
 D-arabitol mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 42.74
-
 D-mannitol mutant enzyme D230A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 49.95
-
 D-mannitol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 55.85
-
 D-arabitol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 68.94
-
 D-mannitol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 93.85
-
 D-mannitol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 94.82
-
 D-arabitol mutant enzyme N300D, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 125.5
-
 D-arabitol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 126.5
-
 D-arabitol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 126.9
-
 D-arabitol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 132.1
-
 D-arabitol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 134.1
-
 D-arabitol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 144.5
-
 D-arabitol mutant enzyme R373A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 145.1
-
 D-arabitol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 176.9
-
 D-arabitol mutant enzyme K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 1800
-
 meso-erythritol Km far above 1800 mM, mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 1800
-
 meso-erythritol Km far above 1800 mM, mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 1800
-
 meso-erythritol Km far above 1800 mM, mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 1800
-
 meso-erythritol Km far above 1800 mM, mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 1800
-
 meso-erythritol Km far above 1800 mM, mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 1800
-
 meso-erythritol Km far above 1800 mM, mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 1800
-
 meso-erythritol Km far above 1800 mM, wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.67 D-mannitol + NAD+ Pseudomonas fluorescens
-
 D-fructose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Pseudomonas fluorescens O08355
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.67 HisPur cobalt resin column chromatography Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-arabitol + NAD+
-
 Pseudomonas fluorescens ?
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Pseudomonas fluorescens D-fructose + NADH + H+
-
 r
1.1.1.67 meso-erythritol + NAD+
-
 Pseudomonas fluorescens ?
-
 ?
1.1.1.67 additional information no activity with glycerol, 1, 2-hexanediol, and 1,2,3-hexanetriol Pseudomonas fluorescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.67 MDH
-
 Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.67 0.00083
-
 D-mannitol mutant enzyme N300D, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.001
-
 D-mannitol mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0012
-
 meso-erythritol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0013
-
 D-arabitol mutant enzyme N300D, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0017
-
 D-arabitol mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0024
-
 meso-erythritol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0029
-
 D-arabitol mutant enzyme D230A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0036
-
 meso-erythritol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0159
-
 meso-erythritol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.018
-
 D-mannitol mutant enzyme R373A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.034
-
 D-arabitol mutant enzyme R373A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.044
-
 D-arabitol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.06
-
 D-arabitol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.092
-
 D-arabitol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.096
-
 D-mannitol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.19
-
 D-mannitol mutant enzyme K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.209
-
 D-mannitol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.21
-
 D-mannitol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.29
-
 D-arabitol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.37
-
 D-arabitol mutant enzyme K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.39
-
 D-mannitol mutant enzyme D230A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.489
-
 meso-erythritol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.49
-
 D-mannitol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.538
-
 meso-erythritol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.599
-
 meso-erythritol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 3.22
-
 D-mannitol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 3.99
-
 D-mannitol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 4.39
-
 D-mannitol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 5.5
-
 D-arabitol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 6.59
-
 D-arabitol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 7.3
-
 D-arabitol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.67 NAD+
-
 Pseudomonas fluorescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.67 0.0000021
-
 meso-erythritol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0000044
-
 meso-erythritol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0000065
-
 meso-erythritol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.000014
-
 D-arabitol mutant enzyme N300D, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.000029
-
 meso-erythritol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00004
-
 D-mannitol mutant enzyme N300D, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.000051
-
 D-arabitol mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00014
-
 D-arabitol mutant enzyme D230A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00016
-
 D-mannitol mutant enzyme H303A/R73A/K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00024
-
 D-arabitol mutant enzyme R373A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00035
-
 D-arabitol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00047
-
 D-arabitol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00069
-
 D-arabitol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00088
-
 meso-erythritol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00097
-
 meso-erythritol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00102
-
 D-mannitol mutant enzyme R373A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.0018
-
 meso-erythritol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00192
-
 D-mannitol mutant enzyme N300S, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.002
-
 D-arabitol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00206
-
 D-arabitol mutant enzyme K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00311
-
 D-mannitol mutant enzyme N300A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00458
-
 D-mannitol mutant enzyme N191A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00522
-
 D-mannitol mutant enzyme H303A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00775
-
 D-mannitol mutant enzyme K381A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.00908
-
 D-mannitol mutant enzyme D230A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.04332
-
 D-arabitol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.05448
-
 D-arabitol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.118
-
 D-arabitol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.215
-
 D-mannitol mutant enzyme E133Q, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.219
-
 D-mannitol mutant enzyme E133A, at pH 7.4 and 25°C Pseudomonas fluorescens
1.1.1.67 0.734
-
 D-mannitol wild type enzyme, at pH 7.4 and 25°C Pseudomonas fluorescens