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Literature summary extracted from

  • Gonaus, C.; Kittl, R.; Sygmund, C.; Haltrich, D.; Peterbauer, C.
    Transcription analysis of pyranose dehydrogenase from the basidiomycete Agaricus bisporus and characterization of the recombinantly expressed enzyme (2016), Protein Expr. Purif., 119, 36-44.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.29 expressed in Pichia pastoris Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.06
-
 2,6-dichlorophenolindophenol with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.1.99.29 0.079
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 0.17
-
 ferrocenium ion with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.1.99.29 0.566
-
 1,4-benzoquinone with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.1.99.29 1.33
-
 L-arabinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 1.54
-
 D-glucose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 2.16
-
 maltose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 2.45
-
 cellobiose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 4.19
-
 D-galactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 4.75
-
 D-xylose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 10.79
-
 raffinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 40.51
-
 lactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 109.1
-
 D-mannose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Agaricus bisporus Q3L1D1
-
-
1.1.99.29 Agaricus bisporus DSM 3056 Q3L1D1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 phenyl Sepharose column chromatography and DEAE-Sepharose column chromatography Agaricus bisporus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.29 mycelium
-
 Agaricus bisporus
-

Storage Stability

EC Number Storage Stability Organism
1.1.99.29 25°C, 100 mM Britton Robinson buffer from pH 4.0-8.0, 1 week, no loss of activity Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 cellobiose + ferrocenium ion
-
 Agaricus bisporus 2-dehydro-cellobiose + ferrocene
-
 ?
1.1.99.29 cellobiose + ferrocenium ion
-
 Agaricus bisporus DSM 3056 2-dehydro-cellobiose + ferrocene
-
 ?
1.1.99.29 D-galactose + ferrocenium ion
-
 Agaricus bisporus 2-dehydro-D-galactose + ferrocene
-
 ?
1.1.99.29 D-galactose + ferrocenium ion
-
 Agaricus bisporus DSM 3056 2-dehydro-D-galactose + ferrocene
-
 ?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
 Agaricus bisporus 2-dehydro-D-glucose + hydroquinone
-
 ?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
 Agaricus bisporus DSM 3056 2-dehydro-D-glucose + hydroquinone
-
 ?
1.1.99.29 D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Agaricus bisporus 2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 ?
1.1.99.29 D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Agaricus bisporus DSM 3056 2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 ?
1.1.99.29 D-glucose + 2,6-dichlorophenolindophenol
-
 Agaricus bisporus 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.29 D-glucose + 2,6-dichlorophenolindophenol
-
 Agaricus bisporus DSM 3056 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.29 D-glucose + ferrocenium ion ferrocenium ion is the preferred electron acceptor Agaricus bisporus 2-dehydro-D-glucose + ferrocene
-
 ?
1.1.99.29 D-mannose + ferrocenium ion
-
 Agaricus bisporus 2-dehydro-D-mannose + ferrocene
-
 ?
1.1.99.29 D-xylose + ferrocenium ion
-
 Agaricus bisporus 2-dehydro-D-xylose + ferrocene
-
 ?
1.1.99.29 L-arabinose + ferrocenium ion
-
 Agaricus bisporus 2-dehydro-L-arabinose + ferrocene
-
 ?
1.1.99.29 lactose + ferrocenium ion
-
 Agaricus bisporus 2-dehydro-lactose + ferrocene
-
 ?
1.1.99.29 maltose + ferrocenium ion
-
 Agaricus bisporus 2-dehydro-maltose + ferrocene
-
 ?
1.1.99.29 raffinose + ferrocenium ion
-
 Agaricus bisporus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 ? x * 75000-100000, recombinant enzyme, SDS-PAGE Agaricus bisporus

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH
-
 Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.29 49
-
-
 Agaricus bisporus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.29 13.31
-
 D-mannose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 15.95
-
 lactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 19.61
-
 raffinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 25.63
-
 cellobiose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 27.26
-
 maltose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 28.7
-
 D-galactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 29.6
-
 L-arabinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 33.7
-
 D-glucose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 36.9
-
 D-xylose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 44.2
-
 1,4-benzoquinone with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.1.99.29 57.6
-
 2,6-dichlorophenolindophenol with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.1.99.29 101.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 111.1
-
 ferrocenium ion with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.29 6
-
-
 Agaricus bisporus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.29 4 8 more than 70% activity between pH 4.0 and 8.0 Agaricus bisporus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.29 6 7.5 after 30 min at 50°C and standard assay pH (7.5) about 10% of initial recombinant enzyme activity is left while at pH 6.0 more than 90% remains active Agaricus bisporus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.29 0.1
-
 D-mannose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 0.4
-
 lactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 1.8
-
 raffinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 6.9
-
 D-galactose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 7.8
-
 D-xylose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 10
-
 cellobiose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 13
-
 maltose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 22
-
 D-glucose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 22
-
 L-arabinose with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 78
-
 1,4-benzoquinone with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.1.99.29 326
-
 ferrocenium ion with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus
1.1.99.29 768
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C Agaricus bisporus
1.1.99.29 958
-
 2,6-dichlorophenolindophenol with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C Agaricus bisporus