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Literature summary extracted from

  • Lacroix Pepin, N.; Chapdelaine, P.; Fortier, M.A.
    Evaluation of the prostaglandin F synthase activity of human and bovine aldo-keto reductases: AKR1A1s complement AKR1B1s as potent PGF synthases (2013), Prostaglandins Other Lipid Mediat., 106, 124-132.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.188 drug development prostaglandin formation is a key component of PGaction and the specific target of non-steroidal anti-inflammatory drugs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 gene AKR1B1, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Bos taurus
1.1.1.188 gene AKR1C3, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.188 9,10-phenanthrenequinone
-
 Bos taurus
1.1.1.188 9,10-phenanthrenequinone
-
 Homo sapiens
1.1.1.188 methylglyoxal
-
 Bos taurus
1.1.1.188 methylglyoxal
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.188 prostaglandin H2 + NADP+ Homo sapiens
-
 prostaglandin F2alpha + NADPH + H+
-
 ?
1.1.1.188 prostaglandin H2 + NADP+ Bos taurus
-
 prostaglandin F2alpha + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Bos taurus P16116
-
-
1.1.1.188 Homo sapiens P15121
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 uterine endometrium
-
 Homo sapiens
-
1.1.1.188 uterine endometrium the bovine endometrium produces huge amounts of PGF2alpha Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.188 0.041
-
 purified recombinant enzyme, pH 7.0, 25°C, in presence of 0.05 mM 9,10-phenanthrenequinone Bos taurus
1.1.1.188 0.078
-
 purified recombinant enzyme, pH 7.0, 25°C, in presence of 0.05 mM 9,10-phenanthrenequinone Homo sapiens
1.1.1.188 0.2
-
 about, purified recombinant enzyme, pH 7.0, 25°C Homo sapiens
1.1.1.188 0.41
-
 about, purified recombinant enzyme, pH 7.0, 25°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 additional information PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview Homo sapiens ?
-
 ?
1.1.1.188 additional information PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview Bos taurus ?
-
 ?
1.1.1.188 prostaglandin H2 + NADP+
-
 Homo sapiens prostaglandin F2alpha + NADPH + H+
-
 ?
1.1.1.188 prostaglandin H2 + NADP+
-
 Bos taurus prostaglandin F2alpha + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.188 AKR1B1
-
 Bos taurus
1.1.1.188 AKR1C3
-
 Homo sapiens
1.1.1.188 More cf. EC 1.1.1.21 Homo sapiens
1.1.1.188 PGFS
-
 Homo sapiens
1.1.1.188 PGFS
-
 Bos taurus
1.1.1.188 prostaglandin F synthase
-
 Homo sapiens
1.1.1.188 prostaglandin F synthase
-
 Bos taurus
1.1.1.188 prostaglandin F2alpha synthase
-
 Homo sapiens
1.1.1.188 prostaglandin F2alpha synthase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.188 25
-
 assay at Homo sapiens
1.1.1.188 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 7
-
 assay at Homo sapiens
1.1.1.188 7
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADP+
-
 Homo sapiens
1.1.1.188 NADP+
-
 Bos taurus

Expression

EC Number Organism Comment Expression
1.1.1.188 Bos taurus AKR1B1 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha up
1.1.1.188 Homo sapiens AKR1C3 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha up

General Information

EC Number General Information Comment Organism
1.1.1.188 metabolism AKR1B1 is able to produce PGF2alpha in the endometrium in addition toAKR1C3. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3 Bos taurus
1.1.1.188 metabolism AKR1C3 is able to produce PGF2alpha in the endometrium in addition to AKR1B1. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3 Homo sapiens
1.1.1.188 additional information comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview Homo sapiens
1.1.1.188 additional information comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview Bos taurus
1.1.1.188 physiological function PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of AKR1C3 in the formation of PGF2alpha from PGH2 in the bovine endometrium Homo sapiens
1.1.1.188 physiological function PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of bovine AKR1B1, previously known as AKR1B5, in the formation of PGF2alpha from PGH2 in the bovine endometrium Bos taurus