BRENDA - Enzyme Database show

Evaluation of the prostaglandin F synthase activity of human and bovine aldo-keto reductases: AKR1A1s complement AKR1B1s as potent PGF synthases

Lacroix Pepin, N.; Chapdelaine, P.; Fortier, M.A.; Prostaglandins Other Lipid Mediat. 106, 124-132 (2013)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.1.1.188
drug development
prostaglandin formation is a key component of PGaction and the specific target of non-steroidal anti-inflammatory drugs
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
gene AKR1B1, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Bos taurus
1.1.1.188
gene AKR1C3, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
9,10-phenanthrenequinone
-
Bos taurus
1.1.1.188
9,10-phenanthrenequinone
-
Homo sapiens
1.1.1.188
methylglyoxal
-
Bos taurus
1.1.1.188
methylglyoxal
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.188
prostaglandin H2 + NADP+
Homo sapiens
-
prostaglandin F2alpha + NADPH + H+
-
-
?
1.1.1.188
prostaglandin H2 + NADP+
Bos taurus
-
prostaglandin F2alpha + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
P16116
-
-
1.1.1.188
Homo sapiens
P15121
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
uterine endometrium
the bovine endometrium produces huge amounts of PGF2alpha
Bos taurus
-
1.1.1.188
uterine endometrium
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.188
0.041
-
purified recombinant enzyme, pH 7.0, 25C, in presence of 0.05 mM 9,10-phenanthrenequinone
Bos taurus
1.1.1.188
0.078
-
purified recombinant enzyme, pH 7.0, 25C, in presence of 0.05 mM 9,10-phenanthrenequinone
Homo sapiens
1.1.1.188
0.2
-
about, purified recombinant enzyme, pH 7.0, 25C
Homo sapiens
1.1.1.188
0.41
-
about, purified recombinant enzyme, pH 7.0, 25C
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
741357
Homo sapiens
?
-
-
-
-
1.1.1.188
additional information
PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
741357
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADP+
-
741357
Homo sapiens
prostaglandin F2alpha + NADPH + H+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADP+
-
741357
Bos taurus
prostaglandin F2alpha + NADPH + H+
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.188
25
-
assay at
Bos taurus
1.1.1.188
25
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
7
-
assay at
Bos taurus
1.1.1.188
7
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADP+
-
Bos taurus
1.1.1.188
NADP+
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
1.1.1.188
drug development
prostaglandin formation is a key component of PGaction and the specific target of non-steroidal anti-inflammatory drugs
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
gene AKR1B1, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Bos taurus
1.1.1.188
gene AKR1C3, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADP+
-
Bos taurus
1.1.1.188
NADP+
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
9,10-phenanthrenequinone
-
Bos taurus
1.1.1.188
9,10-phenanthrenequinone
-
Homo sapiens
1.1.1.188
methylglyoxal
-
Bos taurus
1.1.1.188
methylglyoxal
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.188
prostaglandin H2 + NADP+
Homo sapiens
-
prostaglandin F2alpha + NADPH + H+
-
-
?
1.1.1.188
prostaglandin H2 + NADP+
Bos taurus
-
prostaglandin F2alpha + NADPH + H+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
uterine endometrium
the bovine endometrium produces huge amounts of PGF2alpha
Bos taurus
-
1.1.1.188
uterine endometrium
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.188
0.041
-
purified recombinant enzyme, pH 7.0, 25C, in presence of 0.05 mM 9,10-phenanthrenequinone
Bos taurus
1.1.1.188
0.078
-
purified recombinant enzyme, pH 7.0, 25C, in presence of 0.05 mM 9,10-phenanthrenequinone
Homo sapiens
1.1.1.188
0.2
-
about, purified recombinant enzyme, pH 7.0, 25C
Homo sapiens
1.1.1.188
0.41
-
about, purified recombinant enzyme, pH 7.0, 25C
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
741357
Homo sapiens
?
-
-
-
-
1.1.1.188
additional information
PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
741357
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADP+
-
741357
Homo sapiens
prostaglandin F2alpha + NADPH + H+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADP+
-
741357
Bos taurus
prostaglandin F2alpha + NADPH + H+
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.188
25
-
assay at
Bos taurus
1.1.1.188
25
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
7
-
assay at
Bos taurus
1.1.1.188
7
-
assay at
Homo sapiens
Expression
EC Number
Organism
Commentary
Expression
1.1.1.188
Bos taurus
AKR1B1 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha
up
1.1.1.188
Homo sapiens
AKR1C3 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha
up
General Information
EC Number
General Information
Commentary
Organism
1.1.1.188
metabolism
AKR1B1 is able to produce PGF2alpha in the endometrium in addition toAKR1C3. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3
Bos taurus
1.1.1.188
metabolism
AKR1C3 is able to produce PGF2alpha in the endometrium in addition to AKR1B1. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3
Homo sapiens
1.1.1.188
additional information
comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
Bos taurus
1.1.1.188
additional information
comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
Homo sapiens
1.1.1.188
physiological function
PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of bovine AKR1B1, previously known as AKR1B5, in the formation of PGF2alpha from PGH2 in the bovine endometrium
Bos taurus
1.1.1.188
physiological function
PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of AKR1C3 in the formation of PGF2alpha from PGH2 in the bovine endometrium
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.188
metabolism
AKR1B1 is able to produce PGF2alpha in the endometrium in addition toAKR1C3. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3
Bos taurus
1.1.1.188
metabolism
AKR1C3 is able to produce PGF2alpha in the endometrium in addition to AKR1B1. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3
Homo sapiens
1.1.1.188
additional information
comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
Bos taurus
1.1.1.188
additional information
comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview
Homo sapiens
1.1.1.188
physiological function
PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of bovine AKR1B1, previously known as AKR1B5, in the formation of PGF2alpha from PGH2 in the bovine endometrium
Bos taurus
1.1.1.188
physiological function
PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of AKR1C3 in the formation of PGF2alpha from PGH2 in the bovine endometrium
Homo sapiens
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.1.1.188
Bos taurus
AKR1B1 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha
up
1.1.1.188
Homo sapiens
AKR1C3 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha
up