Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ai, M.; Wang, F.; Zhang, Y.; Huang, F.
    Purification of pyranose oxidase from the white rot fungus Irpex lacteus and its cooperation with laccase in lignin degradation (2014), Process Biochem., 49, 2191-2198.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.74
-
 D-glucose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 7.73
-
 D-galactose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 25.9
-
 D-xylose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 39.3
-
 L-sorbose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 46.4
-
 maltose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 51.1
-
 D-glucono-1,5-lactone at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 290
-
 cellobiose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 370
-
 D-mannose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 727
-
 D-fructose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 902
-
 sucrose at pH 6.5 and 25°C Irpex lacteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 71000
-
-
 Irpex lacteus
1.1.3.10 266000
-
 gel filtration Irpex lacteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.10 D-glucose + O2 Irpex lacteus preferred substrate 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 Irpex lacteus dft-1 preferred substrate 2-dehydro-D-glucose + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Irpex lacteus
-
-
-
1.1.3.10 Irpex lacteus dft-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration Irpex lacteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.10 mycelium
-
 Irpex lacteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 0.31
-
 unpurified enzyme, at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 8.64
-
 after 27.9fold purification, at pH 6.5 and 25°C Irpex lacteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 cellobiose + O2 17.8% activity compared to D-glucose Irpex lacteus 2-dehydrocellobiose + H2O2
-
 ?
1.1.3.10 D-fructose + O2 12.4% activity compared to D-glucose Irpex lacteus 2-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 D-galactose + O2 38.8% activity compared to D-glucose Irpex lacteus 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-galactose + O2 38.8% activity compared to D-glucose Irpex lacteus dft-1 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucono-1,5-lactone + O2 107% activity compared to D-glucose Irpex lacteus 2-dehydro-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.10 D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Irpex lacteus 2-dehydro-D-glucose + ?
-
 ?
1.1.3.10 D-glucose + O2 preferred substrate Irpex lacteus 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 100% activity Irpex lacteus 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 preferred substrate Irpex lacteus dft-1 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 100% activity Irpex lacteus dft-1 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-mannose + O2 18.4% activity compared to D-glucose Irpex lacteus 2-dehydro-D-mannose + H2O2
-
 ?
1.1.3.10 D-xylose + O2 75.1% activity compared to D-glucose Irpex lacteus 2-dehydro-D-xylose + H2O2
-
 ?
1.1.3.10 L-sorbose + O2 122% activity compared to D-glucose Irpex lacteus 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 L-sorbose + O2 122% activity compared to D-glucose Irpex lacteus dft-1 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 lactose + O2 0.71% activity compared to D-glucose Irpex lacteus 2-dehydrolactose + H2O2
-
 ?
1.1.3.10 maltose + O2 92.2% activity compared to D-glucose Irpex lacteus 2-dehydro-D-maltose + H2O2
-
 ?
1.1.3.10 additional information no activity with D-arabinose Irpex lacteus ?
-
 ?
1.1.3.10 additional information no activity with D-arabinose Irpex lacteus dft-1 ?
-
 ?
1.1.3.10 sucrose + O2 82.8% activity compared to D-glucose Irpex lacteus ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 homotetramer 4 * 71000, SDS-PAGE Irpex lacteus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 C-2 specific pyranose-2-oxidase
-
 Irpex lacteus
1.1.3.10 P2O
-
 Irpex lacteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 55
-
-
 Irpex lacteus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.10 30 65 the enzyme shows less than half of its maximum activity at 30°C and 65°C, respectively Irpex lacteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 60 80 the enzyme remains stable up to 60°C. At 70°C, more than half of its initial activity is retained after 30 min of incubation in phosphate buffer, pH 6.5. At 80°C, it is totally inactivated within 30 min Irpex lacteus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 4.19
-
 D-fructose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 6.01
-
 cellobiose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 6.2
-
 D-mannose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 13.1
-
 D-galactose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 25.4
-
 D-xylose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 27.9
-
 sucrose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 31.1
-
 maltose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 33.8
-
 D-glucose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 36.1
-
 D-glucono-1,5-lactone at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 41.4
-
 L-sorbose at pH 6.5 and 25°C Irpex lacteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 6.5
-
-
 Irpex lacteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.10 5 9 at pH 5.0 in citrate buffer and pH 9.0 in glycine-NaOH buffer, the enzyme displays about 60% of its maximum activity. The activity begins to decline dramatically below pH 5.0 and above pH 9.0 Irpex lacteus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.10 3 11 almost no activity loss occurs in the pH range 5.0-11.0 on incubation for 24 h at 25°C. Nearly half and 80% of its initial activity remains at pH 3.0 and 3.5, respectively Irpex lacteus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.10 0.006
-
 D-fructose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 0.017
-
 D-mannose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 0.021
-
 cellobiose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 0.031
-
 sucrose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 0.67
-
 maltose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 0.71
-
 D-glucono-1,5-lactone at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 0.98
-
 D-xylose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 1.05
-
 L-sorbose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 1.7
-
 D-galactose at pH 6.5 and 25°C Irpex lacteus
1.1.3.10 45.6
-
 D-glucose at pH 6.5 and 25°C Irpex lacteus