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Literature summary extracted from

  • Ohshiro, T.; Morita, N.
    Production and characterization of L-fucose dehydrogenase from newly isolated Acinetobacter sp. strain SA-134 (2014), Prep. Biochem. Biotechnol., 44, 382-391.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.122 Ag+ complete inhibition at 1 mM Acinetobacter sp.
1.1.1.122 Co2+ complete inhibition at 1 mM Acinetobacter sp.
1.1.1.122 Cu2+ complete inhibition at 1 mM Acinetobacter sp.
1.1.1.122 EDTA
-
 Acinetobacter sp.
1.1.1.122 Hg2+ complete inhibition at 1 mM Acinetobacter sp.
1.1.1.122 N-ethylmaleimide
-
 Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.122 1.7
-
 NADP+ at pH 8.0 and 37°C Acinetobacter sp.
1.1.1.122 2.6
-
 L-fucose at pH 8.0 and 37°C Acinetobacter sp.
1.1.1.122 2.7
-
 NAD+ at pH 8.0 and 37°C Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.122 23000
-
 gel filtration Acinetobacter sp.
1.1.1.122 25000
-
 1 * 25000, SDS-PAGE Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.122 L-fucose + NADP+ Acinetobacter sp. 100% activity L-fuconolactone + NADPH + H+
-
 ?
1.1.1.122 L-fucose + NADP+ Acinetobacter sp. SA-134 100% activity L-fuconolactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.122 Acinetobacter sp.
-
-
-
1.1.1.122 Acinetobacter sp. SA-134
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.122 DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.122 2.3
-
 cell-free extract, at pH 8.0 and 37°C Acinetobacter sp.
1.1.1.122 217
-
 after 94.3fold purification, at pH 8.0 and 37°C Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.122 D-arabinose + NADP+ good substrate with 65% activity compared to L-fucose Acinetobacter sp. D-arabinolactone + NADPH + H+
-
 ?
1.1.1.122 D-arabinose + NADP+ good substrate with 65% activity compared to L-fucose Acinetobacter sp. SA-134 D-arabinolactone + NADPH + H+
-
 ?
1.1.1.122 L-fucose + NAD+
-
 Acinetobacter sp. L-fuconolactone + NADH + H+
-
 ?
1.1.1.122 L-fucose + NAD+
-
 Acinetobacter sp. SA-134 L-fuconolactone + NADH + H+
-
 ?
1.1.1.122 L-fucose + NADP+ good substrate Acinetobacter sp. L-fuconolactone + NADPH + H+
-
 ?
1.1.1.122 L-fucose + NADP+ 100% activity Acinetobacter sp. L-fuconolactone + NADPH + H+
-
 ?
1.1.1.122 L-fucose + NADP+ good substrate Acinetobacter sp. SA-134 L-fuconolactone + NADPH + H+
-
 ?
1.1.1.122 L-fucose + NADP+ 100% activity Acinetobacter sp. SA-134 L-fuconolactone + NADPH + H+
-
 ?
1.1.1.122 L-galactose + NADP+ L-galactose is a poor substrate with 11% activity compared to L-fucose Acinetobacter sp. ?
-
 ?
1.1.1.122 additional information no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose Acinetobacter sp. ?
-
 ?
1.1.1.122 additional information no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose Acinetobacter sp. SA-134 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.122 monomer 1 * 25000, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.122 L-fucose dehydrogenase
-
 Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.122 50
-
-
 Acinetobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.122 35
-
 the enzyme is stable below 35°C for 60 min Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.122 8
-
-
 Acinetobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.122 6 10 the enzyme is stable between pH 6.0 and 10.0 for 60 min Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.122 NAD+ the enzyme acts on both NAD+ and NADP+ in similar manner Acinetobacter sp.
1.1.1.122 NADP+ the enzyme acts on both NAD+ and NADP+ in similar manner Acinetobacter sp.