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Literature summary extracted from

  • Koliwer-Brandl, H.; Syson, K.; van de Weerd, R.; Chandra, G.; Appelmelk, B.; Alber, M.; Ioerger, T.; Jacobs, W.J.; Geurtsen, J.; Bornemann, S.; Kalscheuer, R.
    Metabolic network for the biosynthesis of intra- and extracellular alpha-glucans required for virulence of Mycobacterium tuberculosis (2016), PLoS Pathog., 12, e1005768.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.342 expressed in Mycobaterium smegmatis mc2155 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.342 alpha-D-glucose-1-phosphate substrate inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.342 0.046
-
 ADP-alpha-D-glucose with 4.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 0.063
-
 ADP-alpha-D-glucose with 2.5 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 0.121
-
 ADP-alpha-D-glucose with 1.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 0.145
-
 ADP-alpha-D-glucose with 0.25 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 0.578
-
 alpha-D-glucose-1-phosphate with 0.5 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 0.695
-
 alpha-D-glucose-1-phosphate with 4.0 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 0.816
-
 alpha-D-glucose-1-phosphate with 0.1 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.342 ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate Mycobacterium tuberculosis
-
 ADP + alpha-maltose-1-phosphate
-
 ?
2.4.1.342 ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate Mycobacterium tuberculosis H37Rv
-
 ADP + alpha-maltose-1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.342 Mycobacterium tuberculosis P9WMZ1
-
-
2.4.1.342 Mycobacterium tuberculosis H37Rv P9WMZ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.342 HisTrap column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.342 ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate
-
 Mycobacterium tuberculosis ADP + alpha-maltose-1-phosphate
-
 ?
2.4.1.342 ADP-alpha-D-glucose + alpha-D-glucose-1-phosphate
-
 Mycobacterium tuberculosis H37Rv ADP + alpha-maltose-1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.342 glgA
-
 Mycobacterium tuberculosis
2.4.1.342 glgM
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.342 15.4
-
 ADP-alpha-D-glucose with 4.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 19.8
-
 ADP-alpha-D-glucose with 2.5 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 20
-
 ADP-alpha-D-glucose with 0.25 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 29.2
-
 ADP-alpha-D-glucose with 1.0 mM alpha-D-glucose-1-phosphate, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 39
-
 alpha-D-glucose-1-phosphate with 0.1 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 80.6
-
 alpha-D-glucose-1-phosphate with 4.0 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 97.9
-
 alpha-D-glucose-1-phosphate with 0.5 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.342 0.578
-
 alpha-D-glucose-1-phosphate with 0.5 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 0.895
-
 alpha-D-glucose-1-phosphate with 4.0 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis
2.4.1.342 1.45
-
 alpha-D-glucose-1-phosphate with 0.1 mM ADP-alpha-D-glucose, at pH 7.0 and 37°C Mycobacterium tuberculosis