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Literature summary extracted from

  • Vuong, T.V.; Master, E.R.
    Fusion of a xylan-binding module to gluco-oligosaccharide oxidase increases activity and promotes stable immobilization (2014), PLoS ONE, 9, e95170.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.B3 expressed in Pichia pastoris strain KM71H Sarocladium strictum

General Stability

EC Number General Stability Organism
1.1.99.B3 the presence of the xylan-binding module CBM22A of Clostridium thermocellum fused to the N-terminal of the enzyme increases its catalytic activity on mono- and oligo-saccharides by 2-3fold while not affecting binding affinity to these substrates, does not alter the thermostability of the enzyme or reduces substrate inhibition Sarocladium strictum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.B3 76000
-
-
 Sarocladium strictum

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.B3 Sarocladium strictum D7PF15
-
-
1.1.99.B3 Sarocladium strictum CBS 346.70 D7PF15
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.B3 glycoprotein
-
 Sarocladium strictum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.B3 Ni-NTA resin column chromatography Sarocladium strictum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.B3 cellobiose + O2
-
 Sarocladium strictum cellobiono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellobiose + O2
-
 Sarocladium strictum CBS 346.70 cellobiono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellohexaose + O2
-
 Sarocladium strictum cellohexaono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellohexaose + O2
-
 Sarocladium strictum CBS 346.70 cellohexaono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellopentaose + O2
-
 Sarocladium strictum cellopentaono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellopentaose + O2
-
 Sarocladium strictum CBS 346.70 cellopentaono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellotriose + O2
-
 Sarocladium strictum cellotriono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellotriose + O2
-
 Sarocladium strictum CBS 346.70 cellotriono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 D-glucose + O2
-
 Sarocladium strictum D-glucono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 D-glucose + O2
-
 Sarocladium strictum CBS 346.70 D-glucono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 D-xylose + O2
-
 Sarocladium strictum D-xylono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 debranched soluble oat spelt xylan + acceptor
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 soluble oat spelt xylan + acceptor
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 xylobiose + O2 highest activity Sarocladium strictum xylobiono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 xylohexaose + O2
-
 Sarocladium strictum xylohexaono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 xylopentaose + O2
-
 Sarocladium strictum xylopentaono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 xylotriose + O2
-
 Sarocladium strictum xylotriono-1,5-lactone + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.B3 ? x * 76000, calculated from amino acid sequence Sarocladium strictum

Synonyms

EC Number Synonyms Comment Organism
1.1.99.B3 gluco-oligosaccharide oxidase
-
 Sarocladium strictum
1.1.99.B3 GOOX-VN
-
 Sarocladium strictum