Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Brugger, D.; Krondorfer, I.; Shelswell, C.; Huber-Dittes, B.; Haltrich, D.; Peterbauer, C.K.
    Engineering pyranose 2-oxidase for modified oxygen reactivity (2014), PLoS ONE, 9, e109242.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 expressed in Escherichia coli BL21 Star DE3 cells Trametes ochracea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.10 L545C the mutant shows 19% of wild type activity Trametes ochracea
1.1.3.10 L547R the mutant shows 0.99% of wild type activity Trametes ochracea
1.1.3.10 N593C the mutant shows 0.058% of wild type activity Trametes ochracea
1.1.3.10 Q448H the mutant shows 1.5% of wild type activity Trametes ochracea
1.1.3.10 T166R the mutant shows 39% of wild type activity Trametes ochracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.018
-
 D-glucose mutant enzyme L547R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.042
-
 D-glucose mutant enzyme L545C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.046
-
 D-glucose mutant enzyme L545C, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.057
-
 D-glucose mutant enzyme Q448H, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.082
-
 D-glucose mutant enzyme Q448H, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.092
-
 D-glucose mutant enzyme T166R, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.12
-
 D-glucose mutant enzyme L545C, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.13
-
 D-glucose wild type enzyme, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.14
-
 D-glucose mutant enzyme N593C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.14
-
 D-glucose mutant enzyme T166R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.18
-
 D-glucose mutant enzyme N593C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.23
-
 D-glucose mutant enzyme L547R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.24
-
 D-glucose mutant enzyme Q448H, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.24
-
 D-glucose wild type enzyme, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.25
-
 D-glucose wild type enzyme, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.4
-
 D-glucose mutant enzyme L547R, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.56
-
 D-glucose mutant enzyme Q448H, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.72
-
 D-glucose mutant enzyme L545C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.81
-
 D-glucose mutant enzyme N593C, with 1 ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.98
-
 D-glucose mutant enzyme N593C, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 1.6
-
 D-glucose mutant enzyme T166R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 1.7
-
 D-glucose mutant enzyme L547R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 1.9
-
 D-glucose wild type enzyme, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 2.1
-
 D-glucose mutant enzyme T166R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 65000
-
 x * 65000, SDS-PAGE Trametes ochracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.10 D-glucose + O2 Trametes ochracea
-
 2-dehydro-D-glucose + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Trametes ochracea Q7ZA32
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 IMAC Ni-charged resin column chromatography Trametes ochracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-glucose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-glucose + 2,6-dichlorophenolindophenol
-
 Trametes ochracea 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.10 D-glucose + ferricenium ion
-
 Trametes ochracea 2-dehydro-D-glucose + ferrocenium ion
-
 ?
1.1.3.10 D-glucose + O2
-
 Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 ? x * 65000, SDS-PAGE Trametes ochracea

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 glucose 2-oxidase
-
 Trametes ochracea
1.1.3.10 POX
-
 Trametes ochracea
1.1.3.10 pyranose 2-Oxidase
-
 Trametes ochracea
1.1.3.10 pyranose:oxygen 2-oxidoreductase
-
 Trametes ochracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.18
-
 D-glucose mutant enzyme N593C, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 0.38
-
 D-glucose mutant enzyme Q448H, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 1.1
-
 D-glucose mutant enzyme L547R, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 2.7
-
 D-glucose mutant enzyme L545C, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 3.9
-
 D-glucose mutant enzyme N593C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 4.6
-
 D-glucose mutant enzyme N593C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 8.1
-
 D-glucose mutant enzyme Q448H, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 11
-
 D-glucose mutant enzyme T166R, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 12
-
 D-glucose mutant enzyme Q448H, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 22
-
 D-glucose mutant enzyme N593C, with 1 ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 26
-
 D-glucose mutant enzyme L547R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 27
-
 D-glucose mutant enzyme Q448H, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 42
-
 D-glucose wild type enzyme, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 50
-
 D-glucose mutant enzyme L545C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 70
-
 D-glucose wild type enzyme, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 110
-
 D-glucose mutant enzyme L545C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 134
-
 D-glucose mutant enzyme L547R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 134
-
 D-glucose mutant enzyme L547R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 142
-
 D-glucose mutant enzyme L545C, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 151
-
 D-glucose wild type enzyme, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 152
-
 D-glucose wild type enzyme, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 166
-
 D-glucose mutant enzyme T166R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 265
-
 D-glucose mutant enzyme T166R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 442
-
 D-glucose mutant enzyme T166R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 FAD
-
 Trametes ochracea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.10 0.18
-
 D-glucose mutant enzyme N593C, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 2.8
-
 D-glucose mutant enzyme L547R, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 4.6
-
 D-glucose mutant enzyme Q448H, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 22
-
 D-glucose mutant enzyme N593C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 27
-
 D-glucose mutant enzyme N593C, with 1 ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 32
-
 D-glucose mutant enzyme N593C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 34
-
 D-glucose mutant enzyme Q448H, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 38
-
 D-glucose wild type enzyme, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 48
-
 D-glucose mutant enzyme Q448H, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 58
-
 D-glucose mutant enzyme L545C, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 70
-
 D-glucose mutant enzyme L545C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 79
-
 D-glucose mutant enzyme L547R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 120
-
 D-glucose mutant enzyme T166R, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 127
-
 D-glucose mutant enzyme T166R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 203
-
 D-glucose mutant enzyme Q448H, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 281
-
 D-glucose mutant enzyme T166R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 308
-
 D-glucose wild type enzyme, with O2 as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 584
-
 D-glucose mutant enzyme L547R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 592
-
 D-glucose wild type enzyme, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 632
-
 D-glucose wild type enzyme, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 1193
-
 D-glucose mutant enzyme L545C, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 1213
-
 D-glucose mutant enzyme T166R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 1465
-
 D-glucose mutant enzyme L547R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea
1.1.3.10 2608
-
 D-glucose mutant enzyme L545C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C Trametes ochracea