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Literature summary extracted from

  • Kilgore, M.B.; Augustin, M.M.; Starks, C.M.; ONeil-Johnson, M.; May, G.D.; Crow, J.A.; Kutchan, T.M.
    Cloning and characterization of a norbelladine 4-O-methyltransferase involved in the biosynthesis of the Alzheimers drug galanthamine in Narcissus sp. aff. pseudonarcissus (2014), PLoS ONE, 9, e103223.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.336 expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells Narcissus aff. pseudonarcissus MK-2014

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.336 0.0016
-
 norbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 0.0019
-
 N-methylnorbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 0.0073
-
 dopamine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 0.0285
-
 S-adenosyl-L-methionine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.336 27000
-
 x * 27000, calculated from amino acid sequence Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 29000
-
-
 Narcissus aff. pseudonarcissus MK-2014

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.336 S-adenosyl-L-methionine + norbelladine Narcissus aff. pseudonarcissus MK-2014
-
 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.336 Narcissus aff. pseudonarcissus MK-2014 A0A077EWA5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.336 Talon resin column chromatography Narcissus aff. pseudonarcissus MK-2014

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.336 bulb
-
 Narcissus aff. pseudonarcissus MK-2014
-
2.1.1.336 inflorescence
-
 Narcissus aff. pseudonarcissus MK-2014
-
2.1.1.336 leaf
-
 Narcissus aff. pseudonarcissus MK-2014
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.336 additional information no activity with caffeic acid, vanillin, 3,4-dihydroxybenzaldehyde, and tyramine Narcissus aff. pseudonarcissus MK-2014 ?
-
 ?
2.1.1.336 S-adenosyl-L-methionine + dopamine
-
 Narcissus aff. pseudonarcissus MK-2014 ?
-
 ?
2.1.1.336 S-adenosyl-L-methionine + N-methylnorbelladine
-
 Narcissus aff. pseudonarcissus MK-2014 ?
-
 ?
2.1.1.336 S-adenosyl-L-methionine + norbelladine
-
 Narcissus aff. pseudonarcissus MK-2014 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.336 ? x * 27000, calculated from amino acid sequence Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 ? x * 29000, His-tagged enzyme, SDS-PAGE Narcissus aff. pseudonarcissus MK-2014

Synonyms

EC Number Synonyms Comment Organism
2.1.1.336 N4OMT1
-
 Narcissus aff. pseudonarcissus MK-2014

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.336 45
-
-
 Narcissus aff. pseudonarcissus MK-2014

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.336 0.022
-
 norbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 0.043
-
 N-methylnorbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 0.06
-
 dopamine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 0.075
-
 S-adenosyl-L-methionine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.336 8.8
-
-
 Narcissus aff. pseudonarcissus MK-2014

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.336 2.7
-
 S-adenosyl-L-methionine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 8.3
-
 dopamine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 13.3
-
 norbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
2.1.1.336 21.7
-
 N-methylnorbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014