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Literature summary extracted from

  • Takeda, K.; Matsumura, H.; Ishida, T.; Samejima, M.; Ohno, H.; Yoshida, M.; Igarashi, K.; Nakamura, N.
    Characterization of a novel PQQ-dependent quinohemoprotein pyranose dehydrogenase from Coprinopsis cinerea classified into auxiliary activities family 12 in carbohydrate-active enzymes (2015), PLoS ONE, 10, e0115722.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.B5 expressed in Pichia pastoris Coprinopsis cinerea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.B5 7.9
-
 D-glucosone at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 23.1
-
 D-talose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 24.8
-
 L-fucose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 30.3
-
 D-arabinose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 49.7
-
 L-galactose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 66.8
-
 D-Lyxose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 84.7
-
 L-gulose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 236
-
 L-Xylose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 363
-
 L-Glucose at pH 8.5 and 30°C Coprinopsis cinerea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.B5 Ca2+ 1 mM Ca2+ is used in assay conditions Coprinopsis cinerea

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.B5 Coprinopsis cinerea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.B5 D-arabinose + 2,6-dichlorophenolindophenol 47.5% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.B5 D-glucosone + ferricytochrome c 100% activity Coprinopsis cinerea ? + ferrocytochrome c
-
 ?
1.1.2.B5 D-lyxose + 2,6-dichlorophenolindophenol 13.4% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.B5 D-talose + ferricytochrome c 24.8% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
 ?
1.1.2.B5 L-fucose + 2,6-dichlorophenolindophenol 77.7% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.B5 L-galactose + ferricytochrome c 65.4% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
 ?
1.1.2.B5 L-glucose + ferricytochrome c 9.2% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
 ?
1.1.2.B5 L-gulose + 2,6-dichlorophenolindophenol 52.1% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.B5 L-lyxose + 2,6-dichlorophenolindophenol 4.0% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.B5 L-mannose + 2,6-dichlorophenolindophenol 3.6% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.B5 L-rhamnose + 2,6-dichlorophenolindophenol 0.7% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.B5 L-xylose + ferricytochrome c 11.9% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
 ?
1.1.2.B5 additional information the enzyme prefers monosaccharides with equatorial C-2, C-3 hydroxyl groups and an axial C-4 hydroxyl group in the 1C4 chair conformation Coprinopsis cinerea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.B5 PDH
-
 Coprinopsis cinerea
1.1.2.B5 PQQ-dependent quinohemoprotein pyranose dehydrogenase
-
 Coprinopsis cinerea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.B5 12.9
-
 D-Lyxose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 17.8
-
 D-talose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 22.4
-
 L-Xylose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 24.9
-
 L-Glucose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 35.5
-
 D-arabinose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 53.3
-
 L-gulose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 56.1
-
 L-galactose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 56.4
-
 L-fucose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 74.1
-
 D-glucosone at pH 8.5 and 30°C Coprinopsis cinerea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.B5 heme b
-
 Coprinopsis cinerea
1.1.2.B5 pyrroloquinoline quinone
-
 Coprinopsis cinerea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.2.B5 0.07
-
 L-Glucose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 0.09
-
 L-Xylose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 0.19
-
 D-Lyxose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 0.63
-
 L-gulose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 0.77
-
 D-talose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 1.13
-
 L-galactose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 1.17
-
 D-arabinose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 2.27
-
 L-fucose at pH 8.5 and 30°C Coprinopsis cinerea
1.1.2.B5 9.37
-
 D-glucosone at pH 8.5 and 30°C Coprinopsis cinerea