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Literature summary extracted from

  • Zdunek-Zastocka, E.; Sobczak, M.
    Expression of Pisum sativum PsAO3 gene, which encodes an aldehyde oxidase utilizing abscisic aldehyde, is induced under progressively but not rapidly imposed drought stress (2013), Plant Physiol. Biochem., 71, 57-66.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.14 expressed in Pichia pastoris strain KM71H Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.14 diphenyleneiodonium 16% residual activity at 0.05 mM Pisum sativum
1.2.3.14 estradiol 60% residual activity at 0.1 mM Pisum sativum
1.2.3.14 menadione 78% residual activity at 0.1 mM Pisum sativum
1.2.3.14 methanol complete inhibition at 2% (v/v) Pisum sativum
1.2.3.14 additional information not inhibited by iodoacetamide and ethanol Pisum sativum
1.2.3.14 p-hydroxymercuribenzoate 62% residual activity at 0.1 mM Pisum sativum
1.2.3.14 potassium cyanide 13% residual activity at 1 mM Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.14 0.0051
-
 abscisic aldehyde at pH 7.5 and 30°C Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.14 150000
-
 2 * 150000, SDS-PAGE Pisum sativum
1.2.3.14 302000
-
 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.3.14 abscisic aldehyde + H2O + O2 Pisum sativum
-
 abscisate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.14 Pisum sativum B0LB01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.14 DEAE-Sepharose column chromatography, Ni-IDA resin column chromatography, and Superdex 200 gel filtration Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.14 leaf
-
 Pisum sativum
-
1.2.3.14 root
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.14 abscisic aldehyde + H2O + O2
-
 Pisum sativum abscisate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.3.14 homodimer 2 * 150000, SDS-PAGE Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
1.2.3.14 AO3
-
 Pisum sativum

Expression

EC Number Organism Comment Expression
1.2.3.14 Pisum sativum during a progressively (but not rapidly) induced drought, the level of AO3 transcript increases significantly in the roots and leaves up