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Literature summary extracted from

  • Jang, M.; Park, J.; Kim, M.; Lee, S.; Kang, J.; Kim, T.
    Molecular cloning and gene expression of Sinorhizobium meliloti mannitol dehydrogenase in Escherichia coli, and its enzymatic characterization (2013), Korean J. Microbiol. Biotechnol., 41, 153-159.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.67 expressed in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.67 54000
-
 x * 54000, calculated from amino acid sequence Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.67 D-fructose + NADH + H+ Sinorhizobium meliloti
-
 D-mannitol + NAD+
-
 r
1.1.1.67 D-fructose + NADH + H+ Sinorhizobium meliloti KCTC 2353
-
 D-mannitol + NAD+
-
 r
1.1.1.67 D-mannitol + NAD+ Sinorhizobium meliloti
-
 D-fructose + NADH + H+
-
 r
1.1.1.67 D-mannitol + NAD+ Sinorhizobium meliloti KCTC 2353
-
 D-fructose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Sinorhizobium meliloti
-
-
-
1.1.1.67 Sinorhizobium meliloti KCTC 2353
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-fructose + NADH + H+
-
 Sinorhizobium meliloti D-mannitol + NAD+
-
 r
1.1.1.67 D-fructose + NADH + H+
-
 Sinorhizobium meliloti KCTC 2353 D-mannitol + NAD+
-
 r
1.1.1.67 D-mannitol + NAD+
-
 Sinorhizobium meliloti D-fructose + NADH + H+
-
 r
1.1.1.67 D-mannitol + NAD+
-
 Sinorhizobium meliloti KCTC 2353 D-fructose + NADH + H+
-
 r
1.1.1.67 additional information no activity with NADP+ and NADPH Sinorhizobium meliloti ?
-
 ?
1.1.1.67 additional information no activity with NADP+ and NADPH Sinorhizobium meliloti KCTC 2353 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.67 ? x * 54000, calculated from amino acid sequence Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
1.1.1.67 MDH
-
 Sinorhizobium meliloti
1.1.1.67 NAD+-dependent mannitol dehydrogenase
-
 Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.67 7
-
 optimum pH for D-fructose reduction Sinorhizobium meliloti
1.1.1.67 10
-
 optimum pH for D-mannitol oxidation Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.67 NAD+
-
 Sinorhizobium meliloti