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Literature summary extracted from

  • Wang, J.; Chen, S.; Liao, Y.; Zhang, E.; Feng, S.; Yu, S.; Li, L.F.; He, W.R.; Li, Y.; Luo, Y.; Sun, Y.; Zhou, M.; Wang, X.; Munir, M.; Li, S.; Qiu, H.J.
    Mitogen-activated protein kinase kinase 2 (MEK2), a novel E2-interacting protein, promotes the growth of classical swine fever virus via attenuation of the JAK-STAT signaling pathway (2016), J. Virol., 90, 10271-10283.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.12.2 recombinant expression of GST-tagged MEK2 mutants in Escherichia coli strain BL21(DE3) and in HEK-293T cells, GST pulldown assay on glutathione resin with recombinantly expressed FLAG-tagged E2 protein Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.12.2 additional information construction of a stable PK-15 cell line with MEK2 knockdown by expression of specific shRNA Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.12.2 additional information no inhibition of MEK2 by MEK1/ERK1/2-specific inhibitor PD98059 Sus scrofa
2.7.12.2 U0126 a specific inhibitor for MEK1/2/ERK1/2 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.12.2 Mg2+ required Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.12.2 ATP + extracellular regulated kinase 1 Sus scrofa
-
 ADP + phosphorylated extracellular regulated kinase 1
-
 ?
2.7.12.2 ATP + extracellular regulated kinase 2 Sus scrofa
-
 ADP + phosphorylated extracellular regulated kinase 2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.12.2 Sus scrofa I3L5D4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.12.2 PK-15 cell
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.12.2 ATP + extracellular regulated kinase 1
-
 Sus scrofa ADP + phosphorylated extracellular regulated kinase 1
-
 ?
2.7.12.2 ATP + extracellular regulated kinase 2
-
 Sus scrofa ADP + phosphorylated extracellular regulated kinase 2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.12.2 MEK2
-
 Sus scrofa
2.7.12.2 mitogen-activated protein kinase kinase 2
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.12.2 ATP
-
 Sus scrofa

General Information

EC Number General Information Comment Organism
2.7.12.2 malfunction overexpression of MEK2 significantly promotes the replication of classical swine fever virus,CSFV, whereas knockdown of MEK2 by lentivirus-mediated small hairpin RNAs dramatically inhibits CSFV replication Sus scrofa
2.7.12.2 metabolism mitogen-activated protein kinase kinase 2 (MEK2) is a kinase that operates immediately upstream of extracellular regulated kinase 1/2 (ERK1/2) and links to Raf and ERK via phosphorylation. The mitogen-activated protein kinase kinase/extracellular regulated kinase (MEK1/2/ERK1/2) cascade is involved in the replication of several members of the Flaviviridae family, including hepatitis C virus and dengue virus, or of classical swine fever virus, a psetivirus in swine Sus scrofa
2.7.12.2 additional information MEK2 does not affect CSFV replication after blocking the interferon-induced Janus kinase signal transducer and activator of transcription (JAK-STAT) signaling pathway by ruxolitinib, a JAK-STAT-specific inhibitor. U0126 but not PD98059 inhibits CSFV replication Sus scrofa
2.7.12.2 physiological function mitogen-activated protein kinase kinase 2, a novel E2-interacting protein, promotes the growth of classical swine fever virus, CSFV, via attenuation of the JAK-STAT signaling pathway. The MEK2-E2 interaction occurs during the late stage of CSFV replication and requires the C-termini of MEK2, residues 266 to 400, and E2 Sus scrofa