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Literature summary extracted from

  • Chollet, A.; Mourey, L.; Lherbet, C.; Delbot, A.; Julien, S.; Baltas, M.; Bernadou, J.; Pratviel, G.; Maveyraud, L.; Bernardes-Genisson, V.
    Crystal structure of the enoyl-ACP reductase of Mycobacterium tuberculosis (InhA) in the apo-form and in complex with the active metabolite of isoniazid pre-formed by a biomimetic approach (2015), J. Struct. Biol., 190, 328-337.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.9 apoenzyme by the sitting drop vapor diffusion method using 100 mM sodium acetate, 100 mM MES pH 6.5 and 30% (w/v) polyethyleneglycol 400 or enzyme in complex with isoniazid, NAD+ or NADH by the hanging drop vapor diffusion method using 100 mM HEPES pH 7.5, 8-10% (w/v) 2-methyl-2,4-pentanediol, 50 mM sodium citrate pH 6.5 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 isoniazid
-
 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.9 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+ Mycobacterium tuberculosis
-
 an acyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+ Mycobacterium tuberculosis H37Rv
-
 an acyl-[acyl-carrier protein] + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Mycobacterium tuberculosis P9WGR1
-
-
1.3.1.9 Mycobacterium tuberculosis H37Rv P9WGR1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 nickel-chelated His-Trap column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 Mycobacterium tuberculosis an acyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 Mycobacterium tuberculosis H37Rv an acyl-[acyl-carrier protein] + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl-ACP reductase
-
 Mycobacterium tuberculosis
1.3.1.9 InhA
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.9 57.6
-
 melting temperature Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Mycobacterium tuberculosis