EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.33 | expressed in Escherichia coli BL21(DE3) cells | Thermosynechococcus vestitus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.33 | G19A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
1.3.1.33 | H236A | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | K42A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
1.3.1.33 | N149V | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | N39V | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | N90A | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | R38V | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
1.3.1.33 | S16C | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
1.3.1.33 | S189A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
1.3.1.33 | T145A | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | T147F | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | T147S | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | T230A | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
1.3.1.33 | T230F | the mutant shows reduced value for turnover number compared to the wild type enzyme | Thermosynechococcus vestitus |
1.3.1.33 | T230S | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
1.3.1.33 | Y94F | the mutant shows wild type value for turnover number | Thermosynechococcus vestitus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.33 | protochlorophyllide + NADPH + H+ | Thermosynechococcus vestitus | - |
chlorophyllide a + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.33 | Thermosynechococcus vestitus | Q8DLC1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.33 | protochlorophyllide + NADPH + H+ | - |
Thermosynechococcus vestitus | chlorophyllide a + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.33 | light-activated enzyme protochlorophyllide oxidoreductase | - |
Thermosynechococcus vestitus |
1.3.1.33 | POR | - |
Thermosynechococcus vestitus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.33 | 0.01 | - |
protochlorophyllide | mutant enzyme N149V, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.01 | - |
protochlorophyllide | mutant enzyme T145A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.01 | - |
protochlorophyllide | mutant enzyme T147F, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.01 | - |
protochlorophyllide | mutant enzyme T230F, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.02 | - |
protochlorophyllide | mutant enzyme N39V, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.02 | - |
protochlorophyllide | mutant enzyme T147S, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.07 | - |
protochlorophyllide | mutant enzyme N90A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.1 | - |
protochlorophyllide | mutant enzyme H236A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.16 | - |
protochlorophyllide | mutant enzyme G19A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.16 | - |
protochlorophyllide | mutant enzyme K42A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.16 | - |
protochlorophyllide | mutant enzyme R38V, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.16 | - |
protochlorophyllide | mutant enzyme S16C, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.16 | - |
protochlorophyllide | mutant enzyme S189A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.16 | - |
protochlorophyllide | mutant enzyme T230A, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.16 | - |
protochlorophyllide | mutant enzyme T230S, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.17 | - |
protochlorophyllide | wild type enzyme, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus | |
1.3.1.33 | 0.17 | - |
protochlorophyllide | mutant enzyme Y94F, at 25°C, pH not specified in the publication | Thermosynechococcus vestitus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.33 | NADPH | - |
Thermosynechococcus vestitus |