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Literature summary extracted from

  • Yang, Y.; Huang, L.; Wang, J.; Wang, X.; Xu, Z.
    Efficient expression, purification, and characterization of a novel FAD-dependent glucose dehydrogenase from Aspergillus terreus in Pichia pastoris (2014), J. Microbiol. Biotechnol., 24, 1516-1524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 expressed in Pichia pastoris strain GS115 Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.9 89.7
-
 D-glucose at pH 7.5 and 55°C Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Aspergillus terreus Q0CDD9
-
-
1.1.5.9 Aspergillus terreus NIH2624 Q0CDD9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 isopropanol precipitation and nickel affinity column chromatography Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 alpha,alpha-trehalose + 2,6-dichlorophenolindophenol 0.5% activity compared to D-glucose Aspergillus terreus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-galactose + 2,6-dichlorophenolindophenol 2.2% activity compared to D-glucose Aspergillus terreus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-galactose + 2,6-dichlorophenolindophenol 2.2% activity compared to D-glucose Aspergillus terreus NIH2624 D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Aspergillus terreus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Aspergillus terreus NIH2624 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-lactose + 2,6-dichlorophenolindophenol 0.6% activity compared to D-glucose Aspergillus terreus D-lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-lactose + 2,6-dichlorophenolindophenol 0.6% activity compared to D-glucose Aspergillus terreus NIH2624 D-lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol 7.4% activity compared to D-glucose Aspergillus terreus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol 7.4% activity compared to D-glucose Aspergillus terreus NIH2624 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 L-arabinose + 2,6-dichlorophenolindophenol 1.5% activity compared to D-glucose Aspergillus terreus L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltose + 2,6-dichlorophenolindophenol 17.7% activity compared to D-glucose Aspergillus terreus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 additional information no activity with D-fructose, S-sorbitol, and D-sucrose Aspergillus terreus ?
-
 ?
1.1.5.9 additional information no activity with D-fructose, S-sorbitol, and D-sucrose Aspergillus terreus NIH2624 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 ? x * 83000-150000, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD-dependent glucose dehydrogenase
-
 Aspergillus terreus
1.1.5.9 FAD-GDH
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.9 55
-
-
 Aspergillus terreus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.5.9 30 65 about 70% activity at 30°C, about 80% activity at 35°C, about 90% activity at 45°C, about 95% activity at 50°C, 100% activity at 55°C, about 98% activity at 60°C, about 85% activity at 65°C Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.9 60
-
 the enzyme displays very high thermal stability with a half-life of 82 min at 60°C Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.9 7.5
-
-
 Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.5.9 6.5 8.5 more than 70% activity between pH 6.0 and 8.5 Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.5.9 3 9 the enzyme remains stable after 20 min at pH 3.0-9.0 Aspergillus terreus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD
-
 Aspergillus terreus