EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.99.B3 | expression in Escherichia coli | Proteus myxofaciens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.99.B3 | the overall fold resembles that of known amine or amino acid oxidases. An additional alpha+beta subdomain is placed close to the putative transmembrane alpha-helix and to the active-site entrance, an FAD isoalloxazine ring is exposed to solvent, and a large and accessible active site suits to bind large hydrophobic substrates. The enzyme requires substrate-induced conformational changes of part of the active site, particularly in Arg316 and Phe318, to achieve the correct geometry for catalysis | Proteus myxofaciens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.99.B3 | additional information | a deleltion mutant lacking the N-terminal transmembrane alpha-helix, residues 1-27, i almost completely soluble, catalytic activity is below detection | Proteus myxofaciens |
EC Number | General Stability | Organism |
---|---|---|
1.4.99.B3 | crude extract, 50% residual activity after 24 h at 25°C | Proteus myxofaciens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.99.B3 | additional information | no substrate inhibition up to 100 mM L-phenylalanine | Proteus myxofaciens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.B3 | 3.27 | - |
L-phenylalanine | pH 7.5, 25°C | Proteus myxofaciens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.4.99.B3 | membrane | presene of a membrane environment is required for activity | Proteus myxofaciens | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.99.B3 | 44600 | - |
gel filtration, recombinant N-terminal deletion mutant | Proteus myxofaciens |
1.4.99.B3 | 49900 | - |
- |
Proteus myxofaciens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.99.B3 | Proteus myxofaciens | A0A158RFS7 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.99.B3 | 2.9 | - |
pH 7.5, 25°C | Proteus myxofaciens |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.99.B3 | -20°C, 10% glycerol, 1 month, 50% residual activity | Proteus myxofaciens |
1.4.99.B3 | -80°C, 10% glycerol, 1 month, 75% residual activity | Proteus myxofaciens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.99.B3 | L-cysteine + H2O + 2 cytochrome b | - |
Proteus myxofaciens | 2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b | 43% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | L-Dopa + H2O + 2 cytochrome b | - |
Proteus myxofaciens | 3-(3,4-dihydroxyphenyl)-pyruvate + NH3 + 2 reduced cytochrome b | 56.7% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | L-isoleucine + H2O + 2 cytochrome b | - |
Proteus myxofaciens | 3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b | 43% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | L-leucine + H2O + 2 cytochrome b | - |
Proteus myxofaciens | 2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b | 99.2% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | L-methionine + H2O + 2 cytochrome b | - |
Proteus myxofaciens | 4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b | 86% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | L-phenylalanine + H2O + 2 cytochrome b | - |
Proteus myxofaciens | phenylpyruvate + NH3 + 2 reduced cytochrome b | - |
? | |
1.4.99.B3 | L-phenylalanine + H2O + 2,6-dichlorophenolindophenol | - |
Proteus myxofaciens | phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | membrane-free enzyme can be assayed using 2,6-dichlorophenolindophenol as acceptor | ? | |
1.4.99.B3 | L-phenylalanine ethyl ester + H2O + 2 cytochrome b | - |
Proteus myxofaciens | phenylpyruvate ethyl ester + NH3 + 2 reduced cytochrome b | 33% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | L-tryptophan + H2O + 2 cytochrome b | - |
Proteus myxofaciens | 3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + 2 reduced cytochrome b | 60.5% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | L-tyrosine + H2O + 2 cytochrome b | - |
Proteus myxofaciens | 4-hydroxyphenylpyruvate + NH3 + 2 reduced cytochrome b | 13.7% of the activitywith L-phenylalanine | ? | |
1.4.99.B3 | additional information | the enzyme does not use dioxygen to reoxidize reduced FADH2 and does not produce hydrogen peroxide, instead, it uses a cytochrome b-like protein as an electron acceptor. No substrates: D-amino acids | Proteus myxofaciens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.99.B3 | monomer | 1 * 49900, calculated, recombinant N-terminal deletion mutant | Proteus myxofaciens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.99.B3 | L-amino acid deaminase | - |
Proteus myxofaciens |
1.4.99.B3 | LAAD | - |
Proteus myxofaciens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.99.B3 | 50 | - |
- |
Proteus myxofaciens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.99.B3 | 70 | - |
negligible activity | Proteus myxofaciens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.B3 | 8.5 | - |
L-phenylalanine | pH 7.5, 25°C | Proteus myxofaciens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.99.B3 | 7 | 7.5 | - |
Proteus myxofaciens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.99.B3 | 5 | 9 | negligible activity bleow and above | Proteus myxofaciens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.99.B3 | FAD | an FAD isoalloxazine ring is exposed to solvent | Proteus myxofaciens |