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Literature summary extracted from

  • Weber, S.; Salabei, J.K.; Moeller, G.; Kremmer, E.; Bhatnagar, A.; Adamski, J.; Barski, O.A.
    Aldo-keto Reductase 1B15 (AKR1B15): a mitochondrial human aldo-keto reductase with activity toward steroids and 3-keto-acyl-CoA conjugates (2015), J. Biol. Chem., 290, 6531-6545.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.36 gene AKR1B15 located on chromosome 7, cloning of two isoforms: AKR1B15.1 and AKR1B15.2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged isozymes in Escherichia coli strain BL21(DE3), recombinant expression of C- or N-terminally c-Myctagged isozymes in HEK-293 cells Homo sapiens
1.1.1.62 gene AKR1B15 located on chromosome 7, cloning of two isoforms: AKR1B15.1 and AKR1B15.2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged isozymes in Escherichia coli strain BL21(DE3), recombinant expression of C- or N-terminally c-Myc-tagged isozymes in HEK-293 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.36 additional information
-
 additional information Michaelis-Menten kinetics, binding of dinucleotide cofactors to AKR1B15.1, overview Homo sapiens
1.1.1.36 0.0634
-
 acetoacetyl-CoA pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 additional information
-
 additional information Michaelis-Menten kinetics, binding of dinucleotide cofactors to AKR1B15.1, overview Homo sapiens
1.1.1.62 0.0019
-
 DELTA4-3,17-androstendione pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 0.0025
-
 estrone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 0.0028
-
 androsterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 0.0071
-
 testosterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 0.0091
-
 17beta-estradiol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 0.0192
-
 5alpha-androstane 3alpha,17beta-diol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.36 cytosol isozyme AKR1B15.2 Homo sapiens 5829
-
1.1.1.36 mitochondrion isozyme AKR1B15.1 Homo sapiens 5739
-
1.1.1.62 cytosol isozyme AKR1B15.2 Homo sapiens 5829
-
1.1.1.62 mitochondrion isozyme AKR1B15.1 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Homo sapiens
-
 3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.62 17beta-estradiol + NADP+ Homo sapiens
-
 estrone + NADPH + H+
-
 ?
1.1.1.62 5alpha-androstane-3alpha,17beta-diol + NAD+ Homo sapiens
-
 androsterone + NADH + H+
-
 ?
1.1.1.62 testosterone + NAD+ Homo sapiens
-
 DELTA4-3,17-androstendione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Homo sapiens C9JRZ8
-
-
1.1.1.62 Homo sapiens C9JRZ8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 recombinant His-tagged isozymes AKR1B15.1 and AKR1B15.2 isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens
1.1.1.62 recombinant His-tagged isozymes AKR1B15.1 and AKR1B15.2 isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.36 adipose tissue isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.36 brain fetal, very low expression of isozyme AKR1B15.2 Homo sapiens
-
1.1.1.36 heart very low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.36 HEK-293 cell isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.36 additional information isozyme tissue-specific expression analysis, overview Homo sapiens
-
1.1.1.36 ovary low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.36 placenta isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.36 prostate isozyme AKR1B15.1 Homo sapiens
-
1.1.1.36 skeletal muscle isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.36 testis isozyme AKR1B15.1, very low expression of isozyme AKR1B15.2 Homo sapiens
-
1.1.1.36 thymus isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.36 thyroid gland isozyme AKR1B15.2 Homo sapiens
-
1.1.1.36 uterus very low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 adipose tissue isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 brain fetal, very low expression of isozyme AKR1B15.2 Homo sapiens
-
1.1.1.62 heart very low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 HEK-293 cell isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 additional information isozyme tissue-specific expression analysis, overview Homo sapiens
-
1.1.1.62 ovary low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 placenta isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 prostate isozyme AKR1B15.1 Homo sapiens
-
1.1.1.62 skeletal muscle isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 testis isozyme AKR1B15.1, very low expression of isozyme AKR1B15.2 Homo sapiens
-
1.1.1.62 thymus isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-
1.1.1.62 thyroid gland isozyme AKR1B15.2 Homo sapiens
-
1.1.1.62 uterus very low expression of isozymes AKR1B15.1 and AKR1B15.2 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Homo sapiens 3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.62 17beta-estradiol + NADP+
-
 Homo sapiens estrone + NADPH + H+
-
 ?
1.1.1.62 5alpha-androstane-3alpha,17beta-diol + NAD+
-
 Homo sapiens androsterone + NADH + H+
-
 ?
1.1.1.62 androst-4-ene-3,17-dione + NADH + H+
-
 Homo sapiens testosterone + NAD+
-
 ?
1.1.1.62 androsterone + NADH + H+
-
 Homo sapiens 5alpha-androstane-3alpha,17beta-diol + NAD+
-
 ?
1.1.1.62 estrone + NADPH + H+
-
 Homo sapiens 17beta-estradiol + NADP+
-
 ?
1.1.1.62 testosterone + NAD+
-
 Homo sapiens androst-4-ene-3,17-dione + NADH + H+
-
 ?
1.1.1.62 testosterone + NAD+
-
 Homo sapiens DELTA4-3,17-androstendione + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.36 AKR1B15
-
 Homo sapiens
1.1.1.36 aldo-keto reductase 1B15
-
 Homo sapiens
1.1.1.62 AKR1B15
-
 Homo sapiens
1.1.1.62 aldo-keto reductase 1B15
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.36 37
-
 assay at Homo sapiens
1.1.1.62 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.36 0.5
-
 acetoacetyl-CoA pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 0.5
-
 17beta-estradiol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 0.6
-
 testosterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 1
-
 estrone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 1.1
-
 DELTA4-3,17-androstendione pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 1.7
-
 androsterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 3
-
 5alpha-androstane 3alpha,17beta-diol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.36 7.4
-
 assay at Homo sapiens
1.1.1.62 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 additional information very poor activity with NADH Homo sapiens
1.1.1.36 NADPH
-
 Homo sapiens
1.1.1.62 NADP+
-
 Homo sapiens
1.1.1.62 NADPH
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.1.1.36 metabolism AKR1B15.1 is a mitochondrial carbonyl reductase. Two alternatively spliced protein isoforms encoded by the human AKRgene AKR1B15 exist, the AKR1B15.1 isoform catalyzes reduction of steroids and 3-keto-acyl-CoA conjugates Homo sapiens
1.1.1.62 metabolism AKR1B15.1 is a mitochondrial carbonyl reductase. Two alternatively spliced protein isoforms encoded by the human AKRgene AKR1B15 exist, the AKR1B15.1 isoform catalyzes reduction of steroids and 3-keto-acyl-CoA conjugates Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.36 10
-
 acetoacetyl-CoA pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 1
-
 17beta-estradiol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 1.5
-
 testosterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 2.67
-
 5alpha-androstane 3alpha,17beta-diol pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 6.33
-
 estrone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 10
-
 DELTA4-3,17-androstendione pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens
1.1.1.62 11
-
 androsterone pH 7.4, 37°C recombinant His-tagged enzyme Homo sapiens