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Literature summary extracted from

  • Carbone, V.; Schofield, L.R.; Zhang, Y.; Sang, C.; Dey, D.; Hannus, I.M.; Martin, W.F.; Sutherland-Smith, A.J.; Ronimus, R.S.
    Structure and evolution of the archaeal lipid synthesis enzyme sn-glycerol-1-phosphate dehydrogenase (2015), J. Biol. Chem., 290, 21690-21704.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.261 KCl an optimum concentration of about 150 mM KCl produces a more than 400% increase in activity compared with an absence of KCl Methanocaldococcus jannaschii
1.1.1.261 KCl the optimum concentration of about 150 mM KCl produces more than 400% increase in activity Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.261
-
 Methanocaldococcus jannaschii
1.1.1.261 expressed in Escherichia coli Rosetta 2 (DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.261 sitting drop vapor diffusion method, using 30% (w/v) poly(ethylene glycol) methyl ether 550/polyethylene glycol 20000 and 0.1 M HEPES, pH 7.6 Methanocaldococcus jannaschii
1.1.1.261 structure with bound substrate dihydroxyacetone phosphate, product glycerol 1-phosphate, NADPH, and Zn2+ cofactor. The sole role of His226, His247, and Asp148 is to coordinate the active site Zn2+. A pro-R hydride transfer/relay system is in operation during catalysis, mediated by water and completed by the polarization of the carbonyl of dihydroxyacetone phosphate by Zn2+. Residue Asn104 may influence affinity for the coenzyme and the rate of reaction via its interaction with NADPH Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.261 0.0432
-
 NADPH pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 0.0432
-
 NADPH at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 0.141
-
 NADH pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 0.141
-
 NADH at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 1.11
-
 dihydroxyacetone phosphate cosubstrate NADH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 1.11
-
 dihydroxyacetone phosphate with NADH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 2.05
-
 dihydroxyacetone phosphate cosubstrate NADPH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 2.05
-
 dihydroxyacetone phosphate with NADPH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.261 Cd2+ about 20% of the activity with Zn2+ Methanocaldococcus jannaschii
1.1.1.261 Co2+ about 150% of the activity with Zn2+ Methanocaldococcus jannaschii
1.1.1.261 Fe2+ dependent on Methanocaldococcus jannaschii
1.1.1.261 Mg2+ about 20% of the activity with Zn2+ Methanocaldococcus jannaschii
1.1.1.261 Mn2+ about 20% of the activity with Zn2+ Methanocaldococcus jannaschii
1.1.1.261 Zn2+ a single Zn2+ ion is bound in each glycerol-1-phosphate dehydrogenase molecule forming bonds to His226, His247, and Asp148 Methanocaldococcus jannaschii
1.1.1.261 Zn2+ required for activity, 0.1 mM is the optimum concentration Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.261 43000
-
 1 * 43000, SDS-PAGE Methanocaldococcus jannaschii
1.1.1.261 44000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.261 Methanocaldococcus jannaschii Q58122
-
-
1.1.1.261 Methanocaldococcus jannaschii DSM 2661 Q58122
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.261 nickel affinity column chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.261 dihydroxyacetone phosphate + NADH + H+
-
 Methanocaldococcus jannaschii sn-glycerol 1-phosphate + NAD+
-
 r
1.1.1.261 dihydroxyacetone phosphate + NADH + H+
-
 Methanocaldococcus jannaschii DSM 2661 sn-glycerol 1-phosphate + NAD+
-
 r
1.1.1.261 dihydroxyacetone phosphate + NADH + H+
-
 Methanocaldococcus jannaschii sn-glycerol-1-phosphate + NAD+
-
 ?
1.1.1.261 dihydroxyacetone phosphate + NADH + H+
-
 Methanocaldococcus jannaschii DSM 2661 sn-glycerol-1-phosphate + NAD+
-
 ?
1.1.1.261 dihydroxyacetone phosphate + NADPH + H+
-
 Methanocaldococcus jannaschii sn-glycerol 1-phosphate + NADP+
-
 r
1.1.1.261 dihydroxyacetone phosphate + NADPH + H+
-
 Methanocaldococcus jannaschii DSM 2661 sn-glycerol 1-phosphate + NADP+
-
 r
1.1.1.261 dihydroxyacetone phosphate + NADPH + H+
-
 Methanocaldococcus jannaschii sn-glycerol-1-phosphate + NADP+
-
 ?
1.1.1.261 dihydroxyacetone phosphate + NADPH + H+
-
 Methanocaldococcus jannaschii DSM 2661 sn-glycerol-1-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.261 monomer 1 * 43000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.261 EgsA
-
 Methanocaldococcus jannaschii
1.1.1.261 G1PDH
-
 Methanocaldococcus jannaschii
1.1.1.261 NAD(P)H-dependent G1P dehydrogenase
-
 Methanocaldococcus jannaschii
1.1.1.261 sn-glycerol-1-phosphate dehydrogenase
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.261 1.44
-
 dihydroxyacetone phosphate cosubstrate NADH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 1.44
-
 dihydroxyacetone phosphate with NADH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 2.78
-
 dihydroxyacetone phosphate cosubstrate NADPH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 2.78
-
 dihydroxyacetone phosphate with NADPH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 3.23
-
 NADPH pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 3.23
-
 NADPH at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 3.33
-
 NADH pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 3.33
-
 NADH at pH 7.8 and 65°C Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.261 6.5 7.5
-
 Methanocaldococcus jannaschii
1.1.1.261 6.5 7.5 broad Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.261 NADH
-
 Methanocaldococcus jannaschii
1.1.1.261 NADPH
-
 Methanocaldococcus jannaschii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.261 1.29
-
 dihydroxyacetone phosphate cosubstrate NADH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 1.295
-
 dihydroxyacetone phosphate with NADH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 1.36
-
 dihydroxyacetone phosphate cosubstrate NADPH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 1.36
-
 dihydroxyacetone phosphate with NADPH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 23.67
-
 NADH pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 23.67
-
 NADH at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.1.1.261 74.67
-
 NADPH pH 7.0, 50°C Methanocaldococcus jannaschii
1.1.1.261 74.67
-
 NADPH at pH 7.8 and 65°C Methanocaldococcus jannaschii