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Literature summary extracted from

  • Wu, M.; Beckham, G.T.; Larsson, A.M.; Ishida, T.; Kim, S.; Payne, C.M.; Himmel, M.E.; Crowley, M.F.; Horn, S.J.; Westereng, B.; Igarashi, K.; Samejima, M.; Stahlberg, J.; Eijsink, V.G.; Sandgren, M.
    Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium (2013), J. Biol. Chem., 288, 12828-12839.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.54 expression in Pichia pastoris Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.54 to 1.75 A resolution. Stucture reveals a copper-bound active site common to LPMOs, a collection of aromatic and polar residues near the binding surface that may be responsible for regioselectivity, and substantial differences in loop structures near the binding face. Surface analysis reveals energy wells whose spacing seems adapted to the spacing of cellobiose units along a cellulose chain Phanerodontia chrysosporium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.54 Cu2+ type II copper center, which exhibits a hexacoordination Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Phanerodontia chrysosporium H1AE14
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 phosphoric acid swollen cellulose + ascorbate + O2
-
 Phanerodontia chrysosporium C1-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.54 steam-exploded spruce + ascorbate + O2
-
 Phanerodontia chrysosporium C1-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 GH61D
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 Phanerodontia chrysosporium