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Literature summary extracted from

  • Kohn, L.; Kaback, H.
    Mechanisms of active transport in isolated bacterial membrane vesicles (1973), J. Biol. Chem., 248, 7012-7017.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.12 2-hydroxy-3-butynoic acid inactivation Escherichia coli
1.1.5.12 additional information not inhibited by 4-chloromercuribenzoate, N-ethylmaleimide, or 5,5'-dithiobis(2-nitrobenzoic acid) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.12 0.5
-
 D-lactate presence of Triton X-100, pH 7.8, 25°C Escherichia coli
1.1.5.12 1.4
-
 D-lactate pH 7.8, 25°C Escherichia coli
1.1.5.12 21
-
 L-lactate presence of Triton X-100, pH 7.8, 25°C Escherichia coli
1.1.5.12 30
-
 L-lactate pH 7.8, 25°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.12 75000
-
 x * 75000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.12 Escherichia coli
-
-
-
1.1.5.12 Escherichia coli ML 308-225
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.12
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.12 D-lactate + 2,6-dichloroindophenol
-
 Escherichia coli pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 D-lactate + 2,6-dichloroindophenol
-
 Escherichia coli ML 308-225 pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 Escherichia coli pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 ?
1.1.5.12 D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 Escherichia coli ML 308-225 pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 ?
1.1.5.12 L-lactate + 2,6-dichloroindophenol
-
 Escherichia coli pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 L-lactate + 2,6-dichloroindophenol
-
 Escherichia coli ML 308-225 pyruvate + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.12 additional information not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate Escherichia coli ?
-
 ?
1.1.5.12 additional information not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate Escherichia coli ML 308-225 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.12 ? x * 75000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.12 7.5 8
-
 Escherichia coli
1.1.5.12 8 9.5 presence of Triton X-100 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.12 FAD 1 mol of FAD per mol of enzyme Escherichia coli