EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.100 | expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | Vibrio cholerae serotype O1 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.100 | hanging drop vapor diffusion method, using 2.0-2.7 M ammonium sulfate and 0.1 M Tris (pH 9.0) | Vibrio cholerae serotype O1 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.100 | G141A | the mutant shows less than 5% of wild type activity | Vibrio cholerae serotype O1 |
1.1.1.100 | G92A | the mutant shows less than 60% of wild type activity | Vibrio cholerae serotype O1 |
1.1.1.100 | G92D | the mutant shows less than 5% of wild type activity | Vibrio cholerae serotype O1 |
1.1.1.100 | Q152A | the mutant shows less than 10% of wild type activity | Vibrio cholerae serotype O1 |
1.1.1.100 | Y155F | the mutant shows less than 3% of wild type activity | Vibrio cholerae serotype O1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.100 | 25000 | - |
- |
Vibrio cholerae serotype O1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.100 | 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | Vibrio cholerae serotype O1 | - |
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.100 | Vibrio cholerae serotype O1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.100 | Ni-NTA agarose column chromatography and Superdex 200 pg gel filtration | Vibrio cholerae serotype O1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.100 | 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | - |
Vibrio cholerae serotype O1 | (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
1.1.1.100 | acetoacetyl-CoA + NADPH + H+ | - |
Vibrio cholerae serotype O1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.100 | homodimer or homotetramer | 2 or 4 * 25000, SDS-PAGE | Vibrio cholerae serotype O1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.100 | beta-ketoacyl-(acyl carrier protein) reductase | - |
Vibrio cholerae serotype O1 |
1.1.1.100 | FabG | - |
Vibrio cholerae serotype O1 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.100 | NADPH | - |
Vibrio cholerae serotype O1 |