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Literature summary extracted from

  • Somerville, C.C.; Nishino, S.F.; Spain, J.C.
    Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45 (1995), J. Bacteriol., 177, 3837-3842.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.16 hydroxylamine 1 mM, 108% of initial activity Pseudomonas oleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.16 1-Naphthylamine 1 mM, 31% residual activity Pseudomonas oleovorans
1.7.1.16 CuSO4 0.5 mM, 78% residual activity Pseudomonas oleovorans
1.7.1.16 cysteine 1 mM, 87% residual activity Pseudomonas oleovorans
1.7.1.16 dicumarol 0.01 mM, 40% residual activity Pseudomonas oleovorans
1.7.1.16 N-ethylmaleimide 1 mM, 77% residual activity Pseudomonas oleovorans
1.7.1.16 neocuproin 1 mM, 39% residual activity Pseudomonas oleovorans
1.7.1.16 nitrite 1 mM, 77% residual activity Pseudomonas oleovorans
1.7.1.16 p-hydroxymercuribenzoate 1 mM, 46% residual activity Pseudomonas oleovorans
1.7.1.16 potassium cyanate 0.5 mM, 75% residual activity Pseudomonas oleovorans
1.7.1.16 salicyl hydroxamate 1 mM, 6% residual activity Pseudomonas oleovorans
1.7.1.16 Sodium azide 1 mM, 35% residual activity Pseudomonas oleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.16 0.005
-
nitrobenzene temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans
1.7.1.16 0.009
-
menadione temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans
1.7.1.16 0.183
-
NADPH temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.16 30000
-
gel fitration Pseudomonas oleovorans
1.7.1.16 33000
-
1 * 33000, SDS-PAGE Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.16 nitrobenzene + 2 NADPH + 2 H+ Pseudomonas oleovorans
-
N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
1.7.1.16 nitrobenzene + 2 NADPH + 2 H+ Pseudomonas oleovorans JS45
-
N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
1.7.1.16 nitrosobenzene + NADPH + H+ Pseudomonas oleovorans
-
N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?
1.7.1.16 nitrosobenzene + NADPH + H+ Pseudomonas oleovorans JS45
-
N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.16 Pseudomonas oleovorans Q6DLR9
-
-
1.7.1.16 Pseudomonas oleovorans JS45 Q6DLR9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.16
-
Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.16 245
-
temperature not specified in the publication, pH 8.0 Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.16 additional information 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product Pseudomonas oleovorans ?
-
?
1.7.1.16 additional information 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product Pseudomonas oleovorans JS45 ?
-
?
1.7.1.16 nitrobenzene + 2 NADPH + 2 H+
-
Pseudomonas oleovorans N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
1.7.1.16 nitrobenzene + 2 NADPH + 2 H+
-
Pseudomonas oleovorans JS45 N-hydroxyaniline + 2 NADP+ + H2O N-hydroxyaniline is the only product detected ?
1.7.1.16 nitrobenzene + menadione
-
Pseudomonas oleovorans N-hydroxyaniline + menadiol + H2O
-
?
1.7.1.16 nitrobenzene + menadione
-
Pseudomonas oleovorans JS45 N-hydroxyaniline + menadiol + H2O
-
?
1.7.1.16 nitrosobenzene + NADPH + H+
-
Pseudomonas oleovorans N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?
1.7.1.16 nitrosobenzene + NADPH + H+
-
Pseudomonas oleovorans JS45 N-hydroxyaniline + NADP+ + H2O nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme ?

Subunits

EC Number Subunits Comment Organism
1.7.1.16 monomer 1 * 33000, SDS-PAGE Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.16 FMN tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein Pseudomonas oleovorans
1.7.1.16 additional information no cofactor: NADH Pseudomonas oleovorans
1.7.1.16 NADPH
-
Pseudomonas oleovorans