EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.16 | hydroxylamine | 1 mM, 108% of initial activity | Pseudomonas oleovorans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.16 | 1-Naphthylamine | 1 mM, 31% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | CuSO4 | 0.5 mM, 78% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | cysteine | 1 mM, 87% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | dicumarol | 0.01 mM, 40% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | N-ethylmaleimide | 1 mM, 77% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | neocuproin | 1 mM, 39% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | nitrite | 1 mM, 77% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | p-hydroxymercuribenzoate | 1 mM, 46% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | potassium cyanate | 0.5 mM, 75% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | salicyl hydroxamate | 1 mM, 6% residual activity | Pseudomonas oleovorans | |
1.7.1.16 | Sodium azide | 1 mM, 35% residual activity | Pseudomonas oleovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.1.16 | 0.005 | - |
nitrobenzene | temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans | |
1.7.1.16 | 0.009 | - |
menadione | temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans | |
1.7.1.16 | 0.183 | - |
NADPH | temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.1.16 | 30000 | - |
gel fitration | Pseudomonas oleovorans |
1.7.1.16 | 33000 | - |
1 * 33000, SDS-PAGE | Pseudomonas oleovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.16 | nitrobenzene + 2 NADPH + 2 H+ | Pseudomonas oleovorans | - |
N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
1.7.1.16 | nitrobenzene + 2 NADPH + 2 H+ | Pseudomonas oleovorans JS45 | - |
N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
1.7.1.16 | nitrosobenzene + NADPH + H+ | Pseudomonas oleovorans | - |
N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? | |
1.7.1.16 | nitrosobenzene + NADPH + H+ | Pseudomonas oleovorans JS45 | - |
N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.1.16 | Pseudomonas oleovorans | Q6DLR9 | - |
- |
1.7.1.16 | Pseudomonas oleovorans JS45 | Q6DLR9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.1.16 | - |
Pseudomonas oleovorans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.1.16 | 245 | - |
temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.16 | additional information | 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product | Pseudomonas oleovorans | ? | - |
? | |
1.7.1.16 | additional information | 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product | Pseudomonas oleovorans JS45 | ? | - |
? | |
1.7.1.16 | nitrobenzene + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans | N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
1.7.1.16 | nitrobenzene + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans JS45 | N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
1.7.1.16 | nitrobenzene + menadione | - |
Pseudomonas oleovorans | N-hydroxyaniline + menadiol + H2O | - |
? | |
1.7.1.16 | nitrobenzene + menadione | - |
Pseudomonas oleovorans JS45 | N-hydroxyaniline + menadiol + H2O | - |
? | |
1.7.1.16 | nitrosobenzene + NADPH + H+ | - |
Pseudomonas oleovorans | N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? | |
1.7.1.16 | nitrosobenzene + NADPH + H+ | - |
Pseudomonas oleovorans JS45 | N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.1.16 | monomer | 1 * 33000, SDS-PAGE | Pseudomonas oleovorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.16 | FMN | tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein | Pseudomonas oleovorans | |
1.7.1.16 | additional information | no cofactor: NADH | Pseudomonas oleovorans | |
1.7.1.16 | NADPH | - |
Pseudomonas oleovorans |