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Literature summary extracted from

  • Sun, L.D.; Luo, Z.; Hu, W.; Zhuo, M.Q.; Zheng, J.L.; Chen, Q.L.; Liang, X.F.; Xiong, B.X.
    Purification and characterization of 6-phosphogluconate dehydrogenase (6-PGD) from grass carp (Ctenopharyngodon idella) hepatopancreas (2013), Indian J. Biochem. Biophys., 50, 554-561.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 6-phospho-D-gluconate substrate inhibition Ctenopharyngodon idella
1.1.1.44 Al3+
-
 Ctenopharyngodon idella
1.1.1.44 Cd2+ competitive inhibition Ctenopharyngodon idella
1.1.1.44 Cu2+ competitive inhibition Ctenopharyngodon idella
1.1.1.44 NADP+ substrate inhibition Ctenopharyngodon idella
1.1.1.44 NADPH competitive inhibition Ctenopharyngodon idella
1.1.1.44 Zn2+ competitive inhibition Ctenopharyngodon idella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.0052
-
 NADP+ at pH 8.0 and 25°C Ctenopharyngodon idella
1.1.1.44 0.019
-
 6-phospho-D-gluconate at pH 8.0 and 25°C Ctenopharyngodon idella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 57000
-
 2 * 57000, SDS-PAGE Ctenopharyngodon idella

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Ctenopharyngodon idella
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Ctenopharyngodon idella
-
 grass carp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44 2',5'-ADP-Sepharose 4B column chromatography Ctenopharyngodon idella

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 hepatopancreas
-
 Ctenopharyngodon idella
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 0.017
-
 unpurified enzyme, at pH 8.0 and 25°C Ctenopharyngodon idella
1.1.1.44 5.259
-
 after 309fold purification, at pH 8.0 and 25°C Ctenopharyngodon idella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ctenopharyngodon idella D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 homodimer 2 * 57000, SDS-PAGE Ctenopharyngodon idella

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Ctenopharyngodon idella
1.1.1.44 6PGD
-
 Ctenopharyngodon idella

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.44 50
-
-
 Ctenopharyngodon idella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 8
-
-
 Ctenopharyngodon idella

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+ dependent on Ctenopharyngodon idella

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.44 0.0285
-
 Cd2+ at pH 8.0 and 25°C Ctenopharyngodon idella
1.1.1.44 0.032
-
 NADPH at pH 8.0 and 25°C Ctenopharyngodon idella
1.1.1.44 0.084
-
 Cu2+ at pH 8.0 and 25°C Ctenopharyngodon idella
1.1.1.44 0.12
-
 NADP+ at pH 8.0 and 25°C Ctenopharyngodon idella
1.1.1.44 0.144
-
 Zn2+ at pH 8.0 and 25°C Ctenopharyngodon idella
1.1.1.44 2.05
-
 6-phospho-D-gluconate at pH 8.0 and 25°C Ctenopharyngodon idella

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.44 0.045
-
 at pH 8.0 and 25°C Ctenopharyngodon idella Cd2+
1.1.1.44 0.099
-
 at pH 8.0 and 25°C Ctenopharyngodon idella Zn2+
1.1.1.44 0.293
-
 at pH 8.0 and 25°C Ctenopharyngodon idella Cu2+
1.1.1.44 1.526
-
 at pH 8.0 and 25°C Ctenopharyngodon idella Al3+