Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khan, M.S.; Kanwal, B.; Nazir, S.
    Metabolic engineering of the chloroplast genome reveals that the yeast ArDH gene confers enhanced tolerance to salinity and drought in plants (2015), Front. Plant Sci., 6, 725.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.11 agriculture the gene can be expressed in agronomic plants to withstand abiotic stresses Saccharomyces cerevisiae
1.1.1.11 biotechnology the gene can be expressed in agronomic plants to withstand abiotic stresses Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.11 recombinant expression of the yeast enzyme in Nicotiana tabacum using the chloroplast transformation vector pMSK83 harboring aadA and ArDH genes under the control of chloroplast regulatory sequences Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.11 D-ribulose + NADH + H+ Saccharomyces cerevisiae
-
 D-arabinitol + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.11 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.11 D-ribulose + NADH + H+
-
 Saccharomyces cerevisiae D-arabinitol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.11 arabitol dehydrogenase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.11 NADH
-
 Saccharomyces cerevisiae

Expression

EC Number Organism Comment Expression
1.1.1.11 Saccharomyces cerevisiae ArDH enzyme expression in tobacco chloroplasts confers tolerance to NaCl (up to 400 mM). Transgenic plants compared to wild-type survived for only 4-5 weeks on 400 mM NaCl whereas plants remain green and grow normal on concentrations up to 350 mM NaCl. A-week-old seedlings are also challenged with PEG up to 6% in the liquid medium, considering that membranes and proteins are protected under stress conditions due to accumulation of arabitol in chloroplasts. Seedlings are tolerant to 6% PEG, suggesting that ARDH enzyme maintains integrity of membranes in chloroplasts under drought conditions via metabolic engineering additional information