Literature summary extracted from

Harada, A.; Kamimura, N.; Takeuchi, K.; Yu, H.Y.; Masai, E.; Senda, T.
The crystal structure of a new O-demethylase from Sphingobium sp. strain SYK-6 (2017), FEBS J., 284, 1855-1867.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.1.1.341	-	Sphingobium sp.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.1.1.341	structure of apo form and in complex with substrates and tetrahydrofolate. Residues His60 and Tyr247 are essential to the catalytic activity of LigM. Their interactions with the oxygen atom in the methoxy group of vanillate seem to facilitate a methyl moiety transfer to tetrahydrofolate	Sphingobium sp.

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.1.1.341	H60A	loss of catalytic activity, mutant is able to bind both substrates	Sphingobium sp.
2.1.1.341	Y247F	loss of catalytic activity, mutant is able to bind both substrates	Sphingobium sp.
2.1.1.341	Y31A	loss of catalytic activity	Sphingobium sp.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1.1.341	0.078	-	vanillate	wild-type, pH 8.0, 30°C	Sphingobium sp.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.1.1.341	52300	-	-	Sphingobium sp.
2.1.1.341	104000	-	gel filtration	Sphingobium sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.1.341	Sphingobium sp.	G2IQS7	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.1.341	vanillate + tetrahydrofolate	-	Sphingobium sp.	protocatechuate + 5-methyltetrahydrofolate	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.1.1.341	dimer	2 * 52300, calculated	Sphingobium sp.

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1.1.341	0.385	-	vanillate	wild-type, pH 8.0, 30°C	Sphingobium sp.

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
2.1.1.341	7.5	-	-	Sphingobium sp.

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Release 2023.1 (January 2023)