Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fukuda, Y.; Sone, T.; Sakuraba, H.; Araki, T.; Ohshima, T.; Shibata, T.; Yoneda, K.
    A novel NAD(P)H-dependent carbonyl reductase specifically expressed in the thyroidectomized chicken fatty liver: catalytic properties and crystal structure (2015), FEBS J., 282, 3918-3928.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Gallus gallus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.184 molecular replacement, to 1.98 A resolution in presence of NADPH and ethylene glycol Gallus gallus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 Y172A Km value for 9,10-phenanthrenequinone (model substrate) is 12.5 times higher than that for the wild-type enzyme Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.001
-
 estrone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.002
-
 9,10-phenanthrenequinone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.03
-
 prostaglandin E2 pH 6.5, 25°C Gallus gallus
1.1.1.184 0.04
-
 menadione pH 6.5, 25°C Gallus gallus
1.1.1.184 0.04
-
 testosterone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.04
-
 isatin pH 6.5, 25°C Gallus gallus
1.1.1.184 0.05
-
 acetone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.18
-
 p-toluquinone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.44
-
 4-nitrobenzaldehyde pH 6.5, 25°C Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Gallus gallus F1N9C1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 4-nitrobenzaldehyde + NADPH + H+
-
 Gallus gallus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 9,10-phenanthrenequinone + NADPH + H+
-
 Gallus gallus ? + NADP+
-
 ?
1.1.1.184 acetone + NADPH + H+
-
 Gallus gallus 2-propanol + NADP+
-
 ?
1.1.1.184 estrone + NADPH + H+
-
 Gallus gallus estradiol + NADP+
-
 ?
1.1.1.184 isatin + NADPH + H+
-
 Gallus gallus 3-hydroxy-2-oxoindole + NADP+
-
 ?
1.1.1.184 menadione + NADPH + H+
-
 Gallus gallus ? + NADP+
-
 ?
1.1.1.184 p-toluquinone + NADPH + H+
-
 Gallus gallus ? + NADP+
-
 ?
1.1.1.184 prostaglandin E2 + NADPH + H+
-
 Gallus gallus prostaglandin F2alpha + NADP+
-
 ?
1.1.1.184 testosterone + NADPH + H+
-
 Gallus gallus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 LOC415661 locus name Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 40
-
-
 Gallus gallus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.184 50
-
 10 min, 50% residual activity Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 0.18
-
 acetone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.27
-
 menadione pH 6.5, 25°C Gallus gallus
1.1.1.184 0.45
-
 estrone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.6
-
 testosterone pH 6.5, 25°C Gallus gallus
1.1.1.184 0.68
-
 prostaglandin E2 pH 6.5, 25°C Gallus gallus
1.1.1.184 2.33
-
 9,10-phenanthrenequinone pH 6.5, 25°C Gallus gallus
1.1.1.184 2.55
-
 isatin pH 6.5, 25°C Gallus gallus
1.1.1.184 3.28
-
 p-toluquinone pH 6.5, 25°C Gallus gallus
1.1.1.184 3.48
-
 4-nitrobenzaldehyde pH 6.5, 25°C Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.184 6.5
-
-
 Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.184 6.5 9 10 min, 40°C, at least 50% residual activity Gallus gallus