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Literature summary extracted from

  • Mundaca-Uribe, R.; Valencia-Munoz, D.; Castillo Felices, R.; Hinojosa, J.; Bustos, M.; Pena-Farfal, C.
    Bienzymatic biosensor for malic acid based on malate dehydrogenase and transaminase immobilized onto a glassy carbon powder/carbon nanotubes/NAD+ composite electrode (2016), Electroanalysis, 28, 1-7.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.37 diagnostics the enzyme can be used in a biosensor for malic acid detection in fruit juices, based on malate dehydrogenase (MDH), and glutamate oxaloacetate transaminase (GOT) in a powder vitreous carbon, carbon nanotubes and mineral oil, composite matrix Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.37 additional information construction of a bienzymatic biosensor for malic acid based on malate dehydrogenase and transaminase immobilized onto a glassy carbon powder/carbon nanotubes/NAD+ composite electrode. In order to shift the conversion of malate to oxaloacetate, a reaction to convert oxaloacetate to L-aspartate is coupled to the main reaction, by reacting it with glutamic acid in the presence of glutamate oxaloacetate transaminase, GOT. The main reaction shifts to products, thus promoting the conversion of malate, thereby generating NADH, pH 10.0. Method overview Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Sus scrofa
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 commercial preparation
-
 Sus scrofa
-
1.1.1.37 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 malate dehydrogenase
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Sus scrofa