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Literature summary extracted from

  • Li, Y.; Liu, L.; Tian, P.
    NAD(+)-independent aldehyde oxidase catalyzes cofactor balanced 3-hydroxypropionic acid production in Klebsiella pneumoniae (2014), Biotechnol. Lett., 36, 2215-2221.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.3.1 synthesis in shake-flask cultures, recombinant Klebsiella pneumoniae expressing the enzyme produces 0.89 g 3-hydroxypropionate per l, i.e. 3 g 3-hydroxypropionate per l during 24 h fed-batch cultivation in a 5 l bioreactor Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.1 expression in Escherichia coli and Klebsiella pneumoniae Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.1 6.7
-
 3-hydroxypropionaldehyde recombinant cell extract, expression in Klebsiella pneumoniae, pH 7.0, 37°C Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Pseudomonas sp.
-
-
-
1.2.3.1 Pseudomonas sp. AIU 362
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.3.1 16.6
-
 recombinant cell extract, expression in Klebsiella pneumoniae, pH 7.0, 37°C Pseudomonas sp.
1.2.3.1 31.1
-
 recombinant cell extract, expression in Escherichia coli, pH 7.0, 37°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 3-hydroxypropionaldehyde + H2O + O2
-
 Pseudomonas sp. 3-hydroxypropionate + H2O2
-
 ?
1.2.3.1 3-hydroxypropionaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 3-hydroxypropionate + H2O2
-
 ?
1.2.3.1 acetaldehyde + H2O + O2
-
 Pseudomonas sp. acetate + H2O2
-
 ?
1.2.3.1 acetaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 acetate + H2O2
-
 ?
1.2.3.1 butylaldehyde + H2O + O2
-
 Pseudomonas sp. butanoate + H2O2
-
 ?
1.2.3.1 butylaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 butanoate + H2O2
-
 ?
1.2.3.1 glyoxal + H2O + O2
-
 Pseudomonas sp. glyoxylate + H2O2
-
 ?
1.2.3.1 glyoxal + H2O + O2
-
 Pseudomonas sp. AIU 362 glyoxylate + H2O2
-
 ?
1.2.3.1 propionaldehyde + H2O + O2
-
 Pseudomonas sp. propionate + H2O2
-
 ?
1.2.3.1 propionaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 propionate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.3.1 ALOD
-
 Pseudomonas sp.