BRENDA - Enzyme Database show

Biosynthesis and degradation of canine placental prostaglandins: prepartum changes in expression and function of prostaglandin F2?-synthase (PGFS, AKR1C3) and 15-hydroxyprostaglandin dehydrogenase (HPGD)

Gram, A.; Buechler, U.; Boos, A.; Hoffmann, B.; Kowalewski, M.P.; Biol. Reprod. 89, 2-2 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
gene PGFS, quantitative expression analysis, recombinant expression of His-tagged canine-specific PGFS (AKR1C3) in Escherichia coli
Canis lupus familiaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.188
prostaglandin H2 + NADP+
Canis lupus familiaris
-
prostaglandin F2alpha + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.141
Canis lupus familiaris
E2QX67
-
-
1.1.1.188
Canis lupus familiaris
Q5FYA7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
recombinant His-tagged canine-specific PGFS (AKR1C3) from Escherichia coli
Canis lupus familiaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.141
corpus luteum
-
Canis lupus familiaris
-
1.1.1.141
placenta
-
Canis lupus familiaris
-
1.1.1.141
uterus
-
Canis lupus familiaris
-
1.1.1.188
additional information
quantitative enzyme expression analysis during pregnancy and prepartum
Canis lupus familiaris
-
1.1.1.188
trophoblast
-
Canis lupus familiaris
-
1.1.1.188
uterus
utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation. Colocalization of 15-hydroxyprostaglandin dehydrogenase (HPGD) with PGFS (AKR1C3) expression. Uterine PGFS (AKR1C3) is up-regulated postimplantation and declined prepartum
Canis lupus familiaris
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.141
prostaglandin E2 + NAD+
-
740130
Canis lupus familiaris
15-oxoprostaglandin E2 + NADH + H+
-
-
-
?
1.1.1.141
prostaglandin F2alpha + NAD+
-
740130
Canis lupus familiaris
15-oxoprostaglandin F2alpha + NADH + H+
-
-
-
?
1.1.1.188
additional information
no activity towards prostaglandin E2 synthesis
740130
Canis lupus familiaris
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADP+
-
740130
Canis lupus familiaris
prostaglandin F2alpha + NADPH + H+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Canis lupus familiaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
7.5
-
assay at
Canis lupus familiaris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.141
NAD+
-
Canis lupus familiaris
1.1.1.188
NADP+
-
Canis lupus familiaris
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
gene PGFS, quantitative expression analysis, recombinant expression of His-tagged canine-specific PGFS (AKR1C3) in Escherichia coli
Canis lupus familiaris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.141
NAD+
-
Canis lupus familiaris
1.1.1.188
NADP+
-
Canis lupus familiaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.188
prostaglandin H2 + NADP+
Canis lupus familiaris
-
prostaglandin F2alpha + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
recombinant His-tagged canine-specific PGFS (AKR1C3) from Escherichia coli
Canis lupus familiaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.141
corpus luteum
-
Canis lupus familiaris
-
1.1.1.141
placenta
-
Canis lupus familiaris
-
1.1.1.141
uterus
-
Canis lupus familiaris
-
1.1.1.188
additional information
quantitative enzyme expression analysis during pregnancy and prepartum
Canis lupus familiaris
-
1.1.1.188
trophoblast
-
Canis lupus familiaris
-
1.1.1.188
uterus
utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation. Colocalization of 15-hydroxyprostaglandin dehydrogenase (HPGD) with PGFS (AKR1C3) expression. Uterine PGFS (AKR1C3) is up-regulated postimplantation and declined prepartum
Canis lupus familiaris
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.141
prostaglandin E2 + NAD+
-
740130
Canis lupus familiaris
15-oxoprostaglandin E2 + NADH + H+
-
-
-
?
1.1.1.141
prostaglandin F2alpha + NAD+
-
740130
Canis lupus familiaris
15-oxoprostaglandin F2alpha + NADH + H+
-
-
-
?
1.1.1.188
additional information
no activity towards prostaglandin E2 synthesis
740130
Canis lupus familiaris
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADP+
-
740130
Canis lupus familiaris
prostaglandin F2alpha + NADPH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Canis lupus familiaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
7.5
-
assay at
Canis lupus familiaris
Expression
EC Number
Organism
Commentary
Expression
1.1.1.141
Canis lupus familiaris
levels enzyme mRNA increase significantly reaching the highest mRNA levels during the postimplantation period of pregnancy
up
General Information
EC Number
General Information
Commentary
Organism
1.1.1.188
metabolism
availability of prostaglandins can be regulated by changes in 15-hydroxyprostaglandin dehydrogenase, HPGD, an enzyme that catabolizes prostaglandin E2 and prostaglandin F2alpha to their inactive metabolites 13,14-dihydro-15keto-PGF2alpha and 13,14-dihydro-15-keto prostaglandin E2 (PGEM)
Canis lupus familiaris
1.1.1.188
physiological function
the utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation, suggesting a possible role for PGFS (AKR1C3) in the trophoblast invasion
Canis lupus familiaris
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.188
metabolism
availability of prostaglandins can be regulated by changes in 15-hydroxyprostaglandin dehydrogenase, HPGD, an enzyme that catabolizes prostaglandin E2 and prostaglandin F2alpha to their inactive metabolites 13,14-dihydro-15keto-PGF2alpha and 13,14-dihydro-15-keto prostaglandin E2 (PGEM)
Canis lupus familiaris
1.1.1.188
physiological function
the utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation, suggesting a possible role for PGFS (AKR1C3) in the trophoblast invasion
Canis lupus familiaris
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.1.1.141
Canis lupus familiaris
levels enzyme mRNA increase significantly reaching the highest mRNA levels during the postimplantation period of pregnancy
up