Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gram, A.; Buechler, U.; Boos, A.; Hoffmann, B.; Kowalewski, M.P.
    Biosynthesis and degradation of canine placental prostaglandins: prepartum changes in expression and function of prostaglandin F2?-synthase (PGFS, AKR1C3) and 15-hydroxyprostaglandin dehydrogenase (HPGD) (2013), Biol. Reprod., 89, 2-2.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 gene PGFS, quantitative expression analysis, recombinant expression of His-tagged canine-specific PGFS (AKR1C3) in Escherichia coli Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.188 prostaglandin H2 + NADP+ Canis lupus familiaris
-
 prostaglandin F2alpha + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Canis lupus familiaris E2QX67
-
-
1.1.1.188 Canis lupus familiaris Q5FYA7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.188 recombinant His-tagged canine-specific PGFS (AKR1C3) from Escherichia coli Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.141 corpus luteum
-
 Canis lupus familiaris
-
1.1.1.141 placenta
-
 Canis lupus familiaris
-
1.1.1.141 uterus
-
 Canis lupus familiaris
-
1.1.1.188 additional information quantitative enzyme expression analysis during pregnancy and prepartum Canis lupus familiaris
-
1.1.1.188 trophoblast
-
 Canis lupus familiaris
-
1.1.1.188 uterus utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation. Colocalization of 15-hydroxyprostaglandin dehydrogenase (HPGD) with PGFS (AKR1C3) expression. Uterine PGFS (AKR1C3) is up-regulated postimplantation and declined prepartum Canis lupus familiaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.141 prostaglandin E2 + NAD+
-
 Canis lupus familiaris 15-oxoprostaglandin E2 + NADH + H+
-
 ?
1.1.1.141 prostaglandin F2alpha + NAD+
-
 Canis lupus familiaris 15-oxoprostaglandin F2alpha + NADH + H+
-
 ?
1.1.1.188 additional information no activity towards prostaglandin E2 synthesis Canis lupus familiaris ?
-
 ?
1.1.1.188 prostaglandin H2 + NADP+
-
 Canis lupus familiaris prostaglandin F2alpha + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.141 HPGD
-
 Canis lupus familiaris
1.1.1.188 AKR1C3
-
 Canis lupus familiaris
1.1.1.188 PGF2alpha-synthase
-
 Canis lupus familiaris
1.1.1.188 PGFS
-
 Canis lupus familiaris
1.1.1.188 prostaglandin F synthase UniProt Canis lupus familiaris
1.1.1.188 prostaglandin F2alpha-synthase
-
 Canis lupus familiaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.188 37
-
 assay at Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 7.5
-
 assay at Canis lupus familiaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.141 NAD+
-
 Canis lupus familiaris
1.1.1.188 NADP+
-
 Canis lupus familiaris

Expression

EC Number Organism Comment Expression
1.1.1.141 Canis lupus familiaris levels enzyme mRNA increase significantly reaching the highest mRNA levels during the postimplantation period of pregnancy up

General Information

EC Number General Information Comment Organism
1.1.1.188 metabolism availability of prostaglandins can be regulated by changes in 15-hydroxyprostaglandin dehydrogenase, HPGD, an enzyme that catabolizes prostaglandin E2 and prostaglandin F2alpha to their inactive metabolites 13,14-dihydro-15keto-PGF2alpha and 13,14-dihydro-15-keto prostaglandin E2 (PGEM) Canis lupus familiaris
1.1.1.188 physiological function the utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation, suggesting a possible role for PGFS (AKR1C3) in the trophoblast invasion Canis lupus familiaris