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Literature summary extracted from

  • Xun, L.; Belchik, S.M.; Xun, R.; Huang, Y.; Zhou, H.; Sanchez, E.; Kang, C.; Board, P.G.
    S-Glutathionyl-(chloro)hydroquinone reductases: a novel class of glutathione transferases (2010), Biochem. J., 428, 419-427.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.7 expression in Escherichia coli Sphingobium chlorophenolicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.7 0.0044
-
 2-(glutathione-S-yl)-trichloro-p-hydroquinone pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 0.26
-
 dithiothreitol pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 0.93
-
 2-mercaptoethanol pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 1.3
-
 glutathione pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 1.97
-
 L-cysteine pH 6.5, 30°C Sphingobium chlorophenolicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.7 36900
-
-
 Sphingobium chlorophenolicum
1.8.5.7 65000
-
 gel filtration Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone Sphingobium chlorophenolicum
-
 glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 additional information Sphingobium chlorophenolicum PcpF catalyzes glutathione-dependent reduction of glutathionyl-trichloro-p-hydroquinone via a Ping Pong mechanism. First, PcpF reacts with glutathionyl-trichloro-p-hydroquinone to release trichloro-p-hydroquinone and forms a disulfide bond between its Cys53 residue and the glutathione moiety. Then, a glutathione comes in to regenerate PcpF and release glutathione disulfide ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.7 Sphingobium chlorophenolicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.7 2-mercaptoethanol + 2-(glutathione-S-yl)-trichloro-p-hydroquinone
-
 Sphingobium chlorophenolicum glutathionyl 2-mercaptoethanyl disulfide + trichloro-p-hydroquinone
-
 ?
1.8.5.7 dithiothreitol + 2-(glutathione-S-yl)-trichloro-p-hydroquinone
-
 Sphingobium chlorophenolicum glutathionyl dithiothreityl disulfide + trichloro-p-hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Sphingobium chlorophenolicum glutathione disulfide + hydroquinone
-
 ?
1.8.5.7 glutathione + 2-(glutathione-S-yl)-trichloro-p-hydroquinone
-
 Sphingobium chlorophenolicum glutathione disulfide + trichloro-p-hydroquinone
-
 ?
1.8.5.7 glutathione + 3-(glutathione-S-yl)-2,6-dichloro-p-hydroquinone
-
 Sphingobium chlorophenolicum glutathione disulfide + 2,6-dichloro-p-hydroquinone
-
 ?
1.8.5.7 L-cysteine + 2-(glutathione-S-yl)-trichloro-p-hydroquinone
-
 Sphingobium chlorophenolicum glutathionyl cysteinyl disulfide + trichloro-p-hydroquinone
-
 ?
1.8.5.7 additional information PcpF catalyzes glutathione-dependent reduction of glutathionyl-trichloro-p-hydroquinone via a Ping Pong mechanism. First, PcpF reacts with glutathionyl-trichloro-p-hydroquinone to release trichloro-p-hydroquinone and forms a disulfide bond between its Cys53 residue and the glutathione moiety. Then, a glutathione comes in to regenerate PcpF and release glutathione disulfide Sphingobium chlorophenolicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.7 dimer 2 * 36900, calculated Sphingobium chlorophenolicum

Synonyms

EC Number Synonyms Comment Organism
1.8.5.7 pcpF
-
 Sphingobium chlorophenolicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.5.7 0.37
-
 L-cysteine pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 0.89
-
 dithiothreitol pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 1.09
-
 2-mercaptoethanol pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 2.08
-
 glutathione pH 6.5, 30°C Sphingobium chlorophenolicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.5.7 0.19
-
 L-cysteine pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 1.17
-
 2-mercaptoethanol pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 1.6
-
 glutathione pH 6.5, 30°C Sphingobium chlorophenolicum
1.8.5.7 3.43
-
 dithiothreitol pH 6.5, 30°C Sphingobium chlorophenolicum