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Literature summary extracted from

  • Lenhard, B.; Filipic, S.; Landeka, I.; Skrtic, I.; Söll, D.; Weygand-Durasevic, I.
    Defining the active site of yeast seryl-tRNA synthetase. Mutations in motif 2 loop residues affect tRNA-dependent amino acid recognition (1997), J. Biol. Chem., 272, 1136-1141.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11
-
 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.11 additional information mutants with mutagenesis of portion of the SES1 gene encoding the motif 2 loop reavel elevated Km values for Ser and ATP accompanied by decreases in kcat Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 0.00011
-
 tRNASer aminoacylation, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 0.004
-
 ATP ATP-diphosphate exchange Saccharomyces cerevisiae
6.1.1.11 0.01 0.048 ATP ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae
6.1.1.11 0.025
-
 ATP aminoacylation, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 0.06 0.55 ATP aminoacylation, mutant enzymes Saccharomyces cerevisiae
6.1.1.11 0.063
-
 Ser aminoacylation, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 0.17 0.7 Ser aminoacylation, mutant enzymes Saccharomyces cerevisiae
6.1.1.11 0.45
-
 Ser serine-dependent ATP-diphosphate exchange, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 0.45 0.89 Ser serine-dependent ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Saccharomyces cerevisiae
-
 wild-type and mutants with mutagenesis of a portion of the SES1 gene encoding the motif 2 loop
-
6.1.1.11 Thermus thermophilus P34945
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
 Saccharomyces cerevisiae AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer
-
 Thermus thermophilus AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 additional information serine-dependent ATP-diphosphate exchange Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.11 0.03 0.55 Ser aminoacylation, mutant-enzymes Saccharomyces cerevisiae
6.1.1.11 0.031 0.51 ATP aminoacylation, mutant enzymes Saccharomyces cerevisiae
6.1.1.11 0.04 1.97 ATP serine-dependent ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae
6.1.1.11 0.052 2.1 Ser serine-dependent ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae
6.1.1.11 0.057 0.65 tRNASer aminoacylation, mutant enzymes Saccharomyces cerevisiae
6.1.1.11 0.97
-
 Ser aminoacylation, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 0.99
-
 ATP aminoacylation, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 1.1
-
 tRNASer aminoacylation, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 3.5
-
 ATP serine-dependent ATP-diphosphate exchange, wild-type enzyme Saccharomyces cerevisiae
6.1.1.11 3.8
-
 Ser serine-dependent ATP-diphosphate exchange, wild-type enzyme Saccharomyces cerevisiae